Table 1. Relative levels of the XG and... | American Society of Hematology

Table 1.

Relative levels of the XG and CD99 antigens at the surface of transfected RAG clones and somatic hybrid derivatives

Clones	Selection Marker	XG Copies/cell (×10⁻³)	CD99 Copies/cell (×10⁻³)
Single transfectants
XG13	neo^R	30	0
h.XG66	hyg^R	78	0
MIC22	neo^R	0	310
h.MIC11	hyg^R	0	2 500
Double transfectants
XG13-h.MIC15	neo^R + hyg^R	28	2 200
XG13-h.MIC19	neo^R + hyg^R	26	2 000
MIC22-h.XG12	neo^R + hyg^R	34	420
MIC22-h.XG22	neo^R + hyg^R	40	370
Somatic hybrids
XG13 × h.MIC11	neo^R + hyg^R	15	1 800
MIC22 × h.XG66	neo^R + hyg^R	49	705
XG13 × hyg^R	neo^R + hyg^R	21	0
MIC22 × hyg^R	neo^R + hyg^R	0	750
neo^R × h.XG66	neo^R + hyg^R	45	0
neo^R × h.MIC11	neo^R + hyg^R	0	1 900

Clones	Selection Marker	XG Copies/cell (×10⁻³)	CD99 Copies/cell (×10⁻³)
Single transfectants
XG13	neo^R	30	0
h.XG66	hyg^R	78	0
MIC22	neo^R	0	310
h.MIC11	hyg^R	0	2 500
Double transfectants
XG13-h.MIC15	neo^R + hyg^R	28	2 200
XG13-h.MIC19	neo^R + hyg^R	26	2 000
MIC22-h.XG12	neo^R + hyg^R	34	420
MIC22-h.XG22	neo^R + hyg^R	40	370
Somatic hybrids
XG13 × h.MIC11	neo^R + hyg^R	15	1 800
MIC22 × h.XG66	neo^R + hyg^R	49	705
XG13 × hyg^R	neo^R + hyg^R	21	0
MIC22 × hyg^R	neo^R + hyg^R	0	750
neo^R × h.XG66	neo^R + hyg^R	45	0
neo^R × h.MIC11	neo^R + hyg^R	0	1 900

The different clones were obtained as described in “Materials and methods” and “Results.” The somatic hybrids neo^R × h.XG66 and neo^R × h.MIC11 were generated by fusion of single transfectants expressing XG or CD99 and RAG cells transfected with the empty pcDNA3 vector (selected for the neo^R marker). The somatic hybrids XG13 × hyg^Rand MIC22 × hyg^R were generated by fusion of single transfectants expressing XG or CD99 and RAG cells transfected with the empty pcDNA-hyg vector (selected for the hyg^R marker). Results represent the mean of three measurements, each at a different cell passage.

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