Table 2. Molecular analysis of... | American Society of Hematology

Table 2.

Molecular analysis of potentially functional V_H genes in diffuse large B-cell lymphoma (DLBCL)

DLBCL No.	Biopsy Tissue	Most Similar Germline V_H Gene	Percent V_H Gene Homology	FR/CDR	Observed Mutations		Rf	P Value
DLBCL No.	Biopsy Tissue	Most Similar Germline V_H Gene	Percent V_H Gene Homology	FR/CDR	R	S	Rf	P Value
1	LN	1-69	100.0	FR	0	0	0.750	NA
				CDR	0	0	0.778	NA
2	LN	1-69	89.5	FR	10	10	0.750	0.002
				CDR	8	3	0.778	NS
3	LN	1-69	85.4	FR	16	9	0.750	0.003
				CDR	13	5	0.778	0.021
4	Thyroid	1-03	88.8	FR	19	5	0.750	NS
				CDR	7	2	0.812	NS
5	Small intestine	1-03	80.9	FR	24	19	0.750	0.008
				CDR	10	3	0.812	NS
6	Lung	1-f	81.1	FR	27	12	0.755	NS
				CDR	13	3	0.797	NS
7	LN	1-f	92.4	FR	7	12	0.755	0.006
				CDR	2	1	0.797	NS
8	NP	2-05	93.9	FR	6	5	0.743	0.009
				CDR	7	2	0.802	0.042
9	AT	2-05	98.3	FR	1	2	0.743	NS
				CDR	2	0	0.802	NS
10	LN	2-05	90.0	FR	15	4	0.743	NS
				CDR	8	3	0.802	NS
11	LN	2-05	98.9	FR	1	1	0.743	NS
				CDR	1	0	0.802	NS
12	LN	2-70	92.6	FR	8	9	0.738	0.030
				CDR	4	1	0.802	NS
13	MD	2-70	92.9	FR	6	7	0.738	0.005
				CDR	5	3	0.802	NS
14	Parotid	3-23	76.1	FR	24	23	0.744	<0.001
				CDR	14	10	0.778	NS
15	LN	3-23	97.0	FR	3	3	0.744	NS
				CDR	3	0	0.778	NS
16	LN	3-23	87.9	FR	17	9	0.744	NS
				CDR	7	3	0.778	NS
17	Lung	3-23	95.6	FR	4	6	0.744	0.030
				CDR	3	0	0.778	NS
18	MD	3-23	95.2	FR	2	6	0.744	0.001
				CDR	2	4	0.778	NS
19	LN	3-23	89.8	FR	9	6	0.744	0.001
				CDR	11	4	0.778	0.006
20	Stomach	3-33	96.9	FR	3	3	0.739	NS
				CDR	2	1	0.811	NS
21	Tonsil	3-33	97.6	FR	5	0	0.739	NS
				CDR	1	1	0.811	NS
8	NP	3-33	90.1	FR	11	8	0.739	0.020
				CDR	9	1	0.811	0.048
22	LN	3-33	83.3	FR	18	13	0.739	0.002
				CDR	16	2	0.811	0.008
23	Tonsil	3-33	95.9	FR	3	6	0.739	0.015
				CDR	3	0	0.811	NS
24	LN	3-48	87.6	FR	9	12	0.742	<0.001
				CDR	9	5	0.825	NS
25	AT	3-48	87.1	FR	6	10	0.742	<0.001
				CDR	19	3	0.825	<0.001
26	LN	3-48	90.4	FR	8	12	0.742	0.001
				CDR	4	4	0.825	NS
27	LN	3-48	90.1	FR	6	7	0.742	<0.001
				CDR	10	6	0.825	0.021
28	LN	3-49	93.0	FR	12	3	0.746	NS
				CDR	4	2	0.754	NS
29	LN	3-49	95.3	FR	6	5	0.746	NS
				CDR	2	1	0.754	NS
30	Spleen	3-30	87.4	FR	11	7	0.744	<0.001
				CDR	15	4	0.796	0.001
R	S
31	LN	3-30	83.0	FR	21	12	0.744	0.015
				CDR	14	3	0.796	0.038
32	LN	3-11	89.8	FR	14	6	0.740	NS
				CDR	7	3	0.813	NS
33	Spleen	3-11	92.2	FR	4	8	0.740	<0.001
				CDR	8	3	0.813	0.029
34	ST	3-07	96.3	FR	2	6	0.737	0.006
				CDR	2	1	0.831	NS
35	Testis	3-07	84.7	FR	21	13	0.737	NS
				CDR	6	4	0.831	NS
13	MD	3-07	96.3	FR	4	1	0.737	NS
				CDR	5	1	0.831	0.023
36^†	LN	3-66	73.4	FR	37	21	0.741	0.030
				CDR	18	3	0.794	NS
37	MD	3-73	97.0	FR	2	2	0.747	0.023
				CDR	5	0	0.755	0.007
38	LN	4-34	99.0	FR	1	2	0.732	NS
				CDR	0	0	0.818	NS
9	AT	4-34	100.0	FR	0	0	0.732	NA
				CDR	0	0	0.818	NA
39	LN	4-34	91.4	FR	3	16	0.732	<0.001
				CDR	1	5	0.818	NS
40	LN	4-34	83.7	FR	8	6	0.732	NS
				CDR	5	1	0.818	NS
41	Skin	4-34	97.3	FR	4	2	0.732	NS
				CDR	2	0	0.818	NS
42	Skin	4-61	94.3	FR	4	4	0.722	0.005
				CDR	5	4	0.818	NS
43	LN	4-30-4	92.3	FR	10	4	0.728	NS
				CDR	7	2	0.805	NS
44	LN	4-59	88.0	FR	14	10	0.722	0.025
				CDR	9	2	0.821	NS
45	ST	4-59	75.5	FR	44	21	0.722	NS
				CDR	6	2	0.821	NS
46	Testis	4-39	93.3	FR	4	6	0.725	0.001
				CDR	8	2	0.818	0.016
47	LN	4-39	89.2	FR	8	13	0.725	<0.001
				CDR	7	4	0.818	NS
48	LN	4-39	74.7	FR	27	18	0.725	<0.001
				CDR	19	11	0.818	NS
49^*	Stomach	4-39	75.8	FR	22	27	0.725	<0.001
				CDR	15	8	0.818	NS
50	NN	4-39	87.5	FR	14	14	0.725	0.016
				CDR	6	3	0.818	NS
51^*	LN	4-30-1	96.2	FR	5	3	0.728	NS
				CDR	2	1	0.805	NS
12^*	LN	5-51	92.2	FR	12	4	0.767	NS
				CDR	6	1	0.778	NS
52	LN	5-51	93.1	FR	13	2	0.767	NS
				CDR	4	1	0.778	NS
27	LN	5-51	100.0	FR	0	0	0.767	NA
				CDR	0	0	0.778	NA
53	LN	7-04-1	86.1	FR	13	19	0.746	<0.001
				CDR	6	3	0.784	NS

DLBCL No.	Biopsy Tissue	Most Similar Germline V_H Gene	Percent V_H Gene Homology	FR/CDR	Observed Mutations		Rf	P Value
DLBCL No.	Biopsy Tissue	Most Similar Germline V_H Gene	Percent V_H Gene Homology	FR/CDR	R	S	Rf	P Value
1	LN	1-69	100.0	FR	0	0	0.750	NA
				CDR	0	0	0.778	NA
2	LN	1-69	89.5	FR	10	10	0.750	0.002
				CDR	8	3	0.778	NS
3	LN	1-69	85.4	FR	16	9	0.750	0.003
				CDR	13	5	0.778	0.021
4	Thyroid	1-03	88.8	FR	19	5	0.750	NS
				CDR	7	2	0.812	NS
5	Small intestine	1-03	80.9	FR	24	19	0.750	0.008
				CDR	10	3	0.812	NS
6	Lung	1-f	81.1	FR	27	12	0.755	NS
				CDR	13	3	0.797	NS
7	LN	1-f	92.4	FR	7	12	0.755	0.006
				CDR	2	1	0.797	NS
8	NP	2-05	93.9	FR	6	5	0.743	0.009
				CDR	7	2	0.802	0.042
9	AT	2-05	98.3	FR	1	2	0.743	NS
				CDR	2	0	0.802	NS
10	LN	2-05	90.0	FR	15	4	0.743	NS
				CDR	8	3	0.802	NS
11	LN	2-05	98.9	FR	1	1	0.743	NS
				CDR	1	0	0.802	NS
12	LN	2-70	92.6	FR	8	9	0.738	0.030
				CDR	4	1	0.802	NS
13	MD	2-70	92.9	FR	6	7	0.738	0.005
				CDR	5	3	0.802	NS
14	Parotid	3-23	76.1	FR	24	23	0.744	<0.001
				CDR	14	10	0.778	NS
15	LN	3-23	97.0	FR	3	3	0.744	NS
				CDR	3	0	0.778	NS
16	LN	3-23	87.9	FR	17	9	0.744	NS
				CDR	7	3	0.778	NS
17	Lung	3-23	95.6	FR	4	6	0.744	0.030
				CDR	3	0	0.778	NS
18	MD	3-23	95.2	FR	2	6	0.744	0.001
				CDR	2	4	0.778	NS
19	LN	3-23	89.8	FR	9	6	0.744	0.001
				CDR	11	4	0.778	0.006
20	Stomach	3-33	96.9	FR	3	3	0.739	NS
				CDR	2	1	0.811	NS
21	Tonsil	3-33	97.6	FR	5	0	0.739	NS
				CDR	1	1	0.811	NS
8	NP	3-33	90.1	FR	11	8	0.739	0.020
				CDR	9	1	0.811	0.048
22	LN	3-33	83.3	FR	18	13	0.739	0.002
				CDR	16	2	0.811	0.008
23	Tonsil	3-33	95.9	FR	3	6	0.739	0.015
				CDR	3	0	0.811	NS
24	LN	3-48	87.6	FR	9	12	0.742	<0.001
				CDR	9	5	0.825	NS
25	AT	3-48	87.1	FR	6	10	0.742	<0.001
				CDR	19	3	0.825	<0.001
26	LN	3-48	90.4	FR	8	12	0.742	0.001
				CDR	4	4	0.825	NS
27	LN	3-48	90.1	FR	6	7	0.742	<0.001
				CDR	10	6	0.825	0.021
28	LN	3-49	93.0	FR	12	3	0.746	NS
				CDR	4	2	0.754	NS
29	LN	3-49	95.3	FR	6	5	0.746	NS
				CDR	2	1	0.754	NS
30	Spleen	3-30	87.4	FR	11	7	0.744	<0.001
				CDR	15	4	0.796	0.001
R	S
31	LN	3-30	83.0	FR	21	12	0.744	0.015
				CDR	14	3	0.796	0.038
32	LN	3-11	89.8	FR	14	6	0.740	NS
				CDR	7	3	0.813	NS
33	Spleen	3-11	92.2	FR	4	8	0.740	<0.001
				CDR	8	3	0.813	0.029
34	ST	3-07	96.3	FR	2	6	0.737	0.006
				CDR	2	1	0.831	NS
35	Testis	3-07	84.7	FR	21	13	0.737	NS
				CDR	6	4	0.831	NS
13	MD	3-07	96.3	FR	4	1	0.737	NS
				CDR	5	1	0.831	0.023
36^†	LN	3-66	73.4	FR	37	21	0.741	0.030
				CDR	18	3	0.794	NS
37	MD	3-73	97.0	FR	2	2	0.747	0.023
				CDR	5	0	0.755	0.007
38	LN	4-34	99.0	FR	1	2	0.732	NS
				CDR	0	0	0.818	NS
9	AT	4-34	100.0	FR	0	0	0.732	NA
				CDR	0	0	0.818	NA
39	LN	4-34	91.4	FR	3	16	0.732	<0.001
				CDR	1	5	0.818	NS
40	LN	4-34	83.7	FR	8	6	0.732	NS
				CDR	5	1	0.818	NS
41	Skin	4-34	97.3	FR	4	2	0.732	NS
				CDR	2	0	0.818	NS
42	Skin	4-61	94.3	FR	4	4	0.722	0.005
				CDR	5	4	0.818	NS
43	LN	4-30-4	92.3	FR	10	4	0.728	NS
				CDR	7	2	0.805	NS
44	LN	4-59	88.0	FR	14	10	0.722	0.025
				CDR	9	2	0.821	NS
45	ST	4-59	75.5	FR	44	21	0.722	NS
				CDR	6	2	0.821	NS
46	Testis	4-39	93.3	FR	4	6	0.725	0.001
				CDR	8	2	0.818	0.016
47	LN	4-39	89.2	FR	8	13	0.725	<0.001
				CDR	7	4	0.818	NS
48	LN	4-39	74.7	FR	27	18	0.725	<0.001
				CDR	19	11	0.818	NS
49^*	Stomach	4-39	75.8	FR	22	27	0.725	<0.001
				CDR	15	8	0.818	NS
50	NN	4-39	87.5	FR	14	14	0.725	0.016
				CDR	6	3	0.818	NS
51^*	LN	4-30-1	96.2	FR	5	3	0.728	NS
				CDR	2	1	0.805	NS
12^*	LN	5-51	92.2	FR	12	4	0.767	NS
				CDR	6	1	0.778	NS
52	LN	5-51	93.1	FR	13	2	0.767	NS
				CDR	4	1	0.778	NS
27	LN	5-51	100.0	FR	0	0	0.767	NA
				CDR	0	0	0.778	NA
53	LN	7-04-1	86.1	FR	13	19	0.746	<0.001
				CDR	6	3	0.784	NS

Cases in which an additional nonfunctional V_H gene sequence was detected.

†

In this case nucleotide insertions and deletions were detected.

The P value is the probability that excess (for CDR) or scarcity (for FR) of mutations occurred by chance. Statistically nonsignificant values (P > 0.05) are marked as NS. AT indicates abdominal tumor; CDR, complementary determining region; FR, framework region; LN, lymph node; MD, mediastinal tumor; NA, not applicable; NN, tissue not known; NP, nasopharynx; Rf, inherent susceptibility to R mutations of the FR or CDR; ST, soft tissue.

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