Effect of different chemokines on monocyte differentiation and apoptosis
| Stimulus . | MAX1-Positive Cells, % . | Apoptotic Cells, % . | Necrotic Cells, % . |
|---|---|---|---|
| None | 9.3 ± 5.1 | 73.8 ± 10.9 | 7.0 ± 9.6 |
| PF4 | |||
| 4 μmol/L | 85.0 ± 6.2* | 13.1 ± 0.8* | 1.7 ± 2.0 |
| IL-8 | |||
| 10 nm | 14.7 ± 5.5 | 67.0 ± 8.8 | 3.7 ± 4.7 |
| 100 nm | 13.7 ± 4.7 | 69.7 ± 9.8 | 2.6 ± 3.0 |
| 1000 nm | 29.0 ± 16.5 | 32.0 ± 12.4* | 0.8 ± 0.3 |
| IP-10 | |||
| 10 nm | 18.7 ± 9.8 | 64.9 ± 5.2 | 4.4 ± 5.7 |
| 100 nm | 17.0 ± 5.6 | 60.7 ± 17.2 | 3.0 ± 2.9 |
| 1000 nm | 20.7 ± 13.6 | 48.1 ± 7.8 | 6.2 ± 7.7 |
| RANTES | |||
| 10 nm | 19.3 ± 7.8 | 65.5 ± 10.0 | 4.3 ± 5.5 |
| 100 nm | 19.7 ± 10.0 | 67.0 ± 11.7 | 3.1 ± 4.3 |
| 1000 nm | 23.0 ± 18.5 | 47.0 ± 9.9 | 2.6 ± 2.9 |
| Fractalkine | |||
| 10 nm | 14.0 ± 3.0 | 70.7 ± 12.7 | 5.5 ± 8.2 |
| 100 nm | 13.3 ± 4.2 | 68.4 ± 11.9 | 5.0 ± 6.2 |
| 1000 nm | 15.7 ± 8.1 | 61.5 ± 11.3 | 5.6 ± 8.6 |
| Stimulus . | MAX1-Positive Cells, % . | Apoptotic Cells, % . | Necrotic Cells, % . |
|---|---|---|---|
| None | 9.3 ± 5.1 | 73.8 ± 10.9 | 7.0 ± 9.6 |
| PF4 | |||
| 4 μmol/L | 85.0 ± 6.2* | 13.1 ± 0.8* | 1.7 ± 2.0 |
| IL-8 | |||
| 10 nm | 14.7 ± 5.5 | 67.0 ± 8.8 | 3.7 ± 4.7 |
| 100 nm | 13.7 ± 4.7 | 69.7 ± 9.8 | 2.6 ± 3.0 |
| 1000 nm | 29.0 ± 16.5 | 32.0 ± 12.4* | 0.8 ± 0.3 |
| IP-10 | |||
| 10 nm | 18.7 ± 9.8 | 64.9 ± 5.2 | 4.4 ± 5.7 |
| 100 nm | 17.0 ± 5.6 | 60.7 ± 17.2 | 3.0 ± 2.9 |
| 1000 nm | 20.7 ± 13.6 | 48.1 ± 7.8 | 6.2 ± 7.7 |
| RANTES | |||
| 10 nm | 19.3 ± 7.8 | 65.5 ± 10.0 | 4.3 ± 5.5 |
| 100 nm | 19.7 ± 10.0 | 67.0 ± 11.7 | 3.1 ± 4.3 |
| 1000 nm | 23.0 ± 18.5 | 47.0 ± 9.9 | 2.6 ± 2.9 |
| Fractalkine | |||
| 10 nm | 14.0 ± 3.0 | 70.7 ± 12.7 | 5.5 ± 8.2 |
| 100 nm | 13.3 ± 4.2 | 68.4 ± 11.9 | 5.0 ± 6.2 |
| 1000 nm | 15.7 ± 8.1 | 61.5 ± 11.3 | 5.6 ± 8.6 |
PF4 indicates platelet factor 4; IL-8, interleukin 8; IP-10.
Monocytes were cultured for 72 hours in the presence of various chemokines at the concentrations indicated or left untreated. Cells were subsequently analyzed by flow cytometry for carboxypeptidase M/MAX1 expression or stained with annexin-V-fluorescein isothiocyanate (annexin-V-FITC) in combination with propidium iodide. Results are given as the percentage of positively stained cells. Apoptotic and necrotic cells were determined as described in the legend to Figure 4. The data are expressed as mean ± SD of 3 independent experiments, recorded and analyzed with identical instrument settings for all assays.
Indicates statistically significant difference with the untreated control (P < .008).