Table 1. Immunophenotype of BALB/c... | American Society of Hematology

Table 1.

Immunophenotype of BALB/c spleen cell leukocyte populations

Subpopulation	Whole Spleen	2 h Non-adh. APC	2 h Adh. APC	18 h Adh. APC + Platelets
CD4⁺ T cells	12.3 ± 4.8^*	10.6 ± 0.8	3.0 ± 1.2	2.6 ± 1.0
CD8⁺ T cells	6.1 ± 3.0	5.5 ± 1.4	3.0 ± 2.0	1.9 ± 0.4
B220⁺ B cells	17.6 ± 4.5	15.6 ± 6.0	24.1 ± 7.5	32.3 ± 9.2
F4/80 macrophages	4.5 ± 4.0	4.5 ± 1.3	33.2 ± 3.0	26.7 ± 0.9
MHC class I+ (H-2D^d)	49.1 ± 9.7	45.7 ± 11.3	67.4 ± 7.5	67.7 ± 4.5
MHC class II+ (I-A^d/I-E^d)	24.7 ± 6.9	24.3 ± 4.9	63.0 ± 2.1	58.5 ± 3.9
CD45⁺ cells	44.0 ± 0.4	39.4 ± 10.0	56.1 ± 13.6	58.5 ± 9.9
CD45⁺/CD61⁺ cells	< 1.0	< 1.0	1.5 ± 0.1	5.1 ± 0.1^†

Subpopulation	Whole Spleen	2 h Non-adh. APC	2 h Adh. APC	18 h Adh. APC + Platelets
CD4⁺ T cells	12.3 ± 4.8^*	10.6 ± 0.8	3.0 ± 1.2	2.6 ± 1.0
CD8⁺ T cells	6.1 ± 3.0	5.5 ± 1.4	3.0 ± 2.0	1.9 ± 0.4
B220⁺ B cells	17.6 ± 4.5	15.6 ± 6.0	24.1 ± 7.5	32.3 ± 9.2
F4/80 macrophages	4.5 ± 4.0	4.5 ± 1.3	33.2 ± 3.0	26.7 ± 0.9
MHC class I+ (H-2D^d)	49.1 ± 9.7	45.7 ± 11.3	67.4 ± 7.5	67.7 ± 4.5
MHC class II+ (I-A^d/I-E^d)	24.7 ± 6.9	24.3 ± 4.9	63.0 ± 2.1	58.5 ± 3.9
CD45⁺ cells	44.0 ± 0.4	39.4 ± 10.0	56.1 ± 13.6	58.5 ± 9.9
CD45⁺/CD61⁺ cells	< 1.0	< 1.0	1.5 ± 0.1	5.1 ± 0.1^†

Data are expressed as mean percentage ± SD of total cells (n = 3).

CD45⁺/CD61⁺ phenotype was similar if either syngeneic or allogeneic platelets were used to pulse the APC.