Reversal of apoptosis resistance by treatment with VP-16 in combination with BBGC
| Cell Lines . | Treatment . | Apoptotic Cell (%) . |
|---|---|---|
| U937 | none | 3.0 ± 0.1 |
| VP-16 | 39.1 ± 0.4 | |
| VP-16 + BBGC | 40.0 ± 0.3 | |
| BBGC | 5.3 ± 0.3 | |
| UK711 | none | 1.9 ± 0.1 |
| VP-16 | 5.6 ± 0.3 | |
| VP-16 + BBGC | 13.1 ± 1.4 | |
| BBGC | 3.8 ± 0.0 | |
| UK110 | none | 2.3 ± 0.1 |
| VP-16 | 26.4 ± 0.5 | |
| VP-16 + BBGC | 37.5 ± 0.4 | |
| BBGC | 4.3 ± 0.0 |
| Cell Lines . | Treatment . | Apoptotic Cell (%) . |
|---|---|---|
| U937 | none | 3.0 ± 0.1 |
| VP-16 | 39.1 ± 0.4 | |
| VP-16 + BBGC | 40.0 ± 0.3 | |
| BBGC | 5.3 ± 0.3 | |
| UK711 | none | 1.9 ± 0.1 |
| VP-16 | 5.6 ± 0.3 | |
| VP-16 + BBGC | 13.1 ± 1.4 | |
| BBGC | 3.8 ± 0.0 | |
| UK110 | none | 2.3 ± 0.1 |
| VP-16 | 26.4 ± 0.5 | |
| VP-16 + BBGC | 37.5 ± 0.4 | |
| BBGC | 4.3 ± 0.0 |
Cells were treated with 15 μmol/L of VP-16, 15 μmol/L of VP-16 in combination with 12.5 μmol/L BBGC, and 12.5 μmol/L BBGC alone for 4 h. The percentages of apoptotic cells were determined as subdiploid cells by flow cytometric analysis after staining with PI. The means ± S.D. of triplicate cultures are shown.