Description of locus control region (LCR) and globin gene regulatory elements present in each β/γ-globin hybrid transgene and a summary of single-copy and total mice expressing these constructs
| BGT . | 5′HS3 . | Prom . | βivs2 . | β3′enh . | Mice Expressing . | |
|---|---|---|---|---|---|---|
| Single Copy . | Total . | |||||
| 34 | + | β | − | − | 3/6 (0%-74%) | 4/7 |
| 35 | + | β | + | − | 2/3 (0%-39%) | 6/7 |
| 54 | + | β | − | + | 5/6 (0%-76%) | 13/15 |
| 50 | + | β | + | + | 7/7 (40%-109%) | 8/8 |
| 76 | + | γ | + | + | 5/5 (12%-72%) | 17/17 |
| 64 | + | β | Δ | + | 2/4 (0%-22%) | 13/17 |
| BGT . | 5′HS3 . | Prom . | βivs2 . | β3′enh . | Mice Expressing . | |
|---|---|---|---|---|---|---|
| Single Copy . | Total . | |||||
| 34 | + | β | − | − | 3/6 (0%-74%) | 4/7 |
| 35 | + | β | + | − | 2/3 (0%-39%) | 6/7 |
| 54 | + | β | − | + | 5/6 (0%-76%) | 13/15 |
| 50 | + | β | + | + | 7/7 (40%-109%) | 8/8 |
| 76 | + | γ | + | + | 5/5 (12%-72%) | 17/17 |
| 64 | + | β | Δ | + | 2/4 (0%-22%) | 13/17 |
The range of expression in single-copy animals for each construct is listed as a percentage of Aγ-globin transcripts to endogenous mouse β-globin major transcripts on a per copy basis. Prom, promoter (either β or γ); βivs2, β-globin intron 2; β3′enh, β-globin 3′ enhancer; Δ, β-globin intron 2 present but containing a 372-bp AT-rich region deletion.