Kinetic properties of the GST-fused wild-type and mutant (Cys203Tyr) b5Rs
| Enzyme . | Km (NADH) (μmol/L) . | Km (DCIP) (μmol/L) . | Kcat (NADH) (s−1) . | Kcat (DCIP) (s−1) . | Kcat/Km (NADH) (s−1) · (mol/L)−1 . | Kcat/Km (DCIP) (s−1) · (mol/L)−1 . |
|---|---|---|---|---|---|---|
| Wild type | 26 | 68 | 376 | 673 | 1.4 × 107 | 9.9 × 106 |
| Mutant type | 22 | 66 | 368 | 645 | 1.7 × 107 | 9.8 × 106 |
| Enzyme . | Km (NADH) (μmol/L) . | Km (DCIP) (μmol/L) . | Kcat (NADH) (s−1) . | Kcat (DCIP) (s−1) . | Kcat/Km (NADH) (s−1) · (mol/L)−1 . | Kcat/Km (DCIP) (s−1) · (mol/L)−1 . |
|---|---|---|---|---|---|---|
| Wild type | 26 | 68 | 376 | 673 | 1.4 × 107 | 9.9 × 106 |
| Mutant type | 22 | 66 | 368 | 645 | 1.7 × 107 | 9.8 × 106 |