G-CSF–induced HPC mobilization in mixed chimeras
| Tissue . | CFC . | Saline . | G-CSF . | Fold Change . | P Value . |
|---|---|---|---|---|---|
| Bone marrow, | Total | 96 744 ± 13 947 | 130 545 ± 24 709 | 1.3 | <.02 |
| per femur | G418R | 35 719 ± 12 786 | 24 709 ± 5 847 | 0.7 | NS |
| % G418R | 36.5 ± 9.5 | 18.5 ± 3.6 | — | — | |
| Blood, per mL | Total | 150 ± 69 | 1783 ± 650 | 11.9 | <.001 |
| G418R | 71 ± 37 | 421 ± 150 | 5.9 | <.001 | |
| % G418R | 50.0 ± 21.3 | 24.2 ± 6.0 | — | — | |
| Spleen | Total | 26 923 ± 21 220 | 260 800 ± 166 525 | 9.7 | <.01 |
| G418R | 9 213 ± 4 216 | 44 873 ± 26 632 | 4.9 | <.01 | |
| % G418R | 41.2 ± 21.3 | 18.4 ± 4.4 | — | — |
| Tissue . | CFC . | Saline . | G-CSF . | Fold Change . | P Value . |
|---|---|---|---|---|---|
| Bone marrow, | Total | 96 744 ± 13 947 | 130 545 ± 24 709 | 1.3 | <.02 |
| per femur | G418R | 35 719 ± 12 786 | 24 709 ± 5 847 | 0.7 | NS |
| % G418R | 36.5 ± 9.5 | 18.5 ± 3.6 | — | — | |
| Blood, per mL | Total | 150 ± 69 | 1783 ± 650 | 11.9 | <.001 |
| G418R | 71 ± 37 | 421 ± 150 | 5.9 | <.001 | |
| % G418R | 50.0 ± 21.3 | 24.2 ± 6.0 | — | — | |
| Spleen | Total | 26 923 ± 21 220 | 260 800 ± 166 525 | 9.7 | <.01 |
| G418R | 9 213 ± 4 216 | 44 873 ± 26 632 | 4.9 | <.01 | |
| % G418R | 41.2 ± 21.3 | 18.4 ± 4.4 | — | — |
Mixed chimeras in which both wild type and G-CSFR (−/−) hematopoietic cells contributed to hematopoiesis were treated with saline or G-CSF (n = 6 for each).
Colonies that grew in the presence of 1 mg/mL of G418 (G418R) represent G-CSFR–deficient CFCs. Data represent the mean plus or minus SD. The fold change and significance of the difference between saline-treated and G-CSF–treated mice are shown.
NS indicates nonsignificant.