Sequence of primers used in RT-PCR
| Gene . | Primer Name . | Sequence (5′ → 3′) . | Positions . |
|---|---|---|---|
| ICAM-136 | 5I (5′ primer) | GGTGTATGAACTGAGCAATGTGCAAGAAG | 243 → 271 |
| 3I (3′ primer) | AGCACCGTGGTCGTGACCTCAG | 533 → 512 | |
| 3delI (3′ hybrid primer) | GTGACCTCAGTGAGGTTGGCCCGGGGTGC | 521 → 512, 451 → 433 | |
| 5I-PCR (5′ primer) | TAGCCAACCAATGTGCTATTCAAACT | 273 → 298 | |
| VCAM-137 | 5V (5′ primer) | AGATTGGTGACTCCGTCTCATTGACTTG | 113 → 140 |
| 3V (3′ primer) | CTGAACACTTGACTGTGATCGGCTTC | 415 → 390 | |
| 3delV (3′ hybrid primer) | GATCGGCTTCACCTGGATTCCTTTTTCCAA | 399 → 390, 329 → 310 | |
| GAPDH38 | 5G (5′ primer) | GGTTTACATGTTCCAATATGATTCCACCC | 189 → 217 |
| 3G (3′ primer) | GTTGTCATACTTCTCATGGTTCACACCC | 486 → 459 | |
| 3delG (3′ hybrid primer) | GTTCACACCCTGCAAATGAGCCCCA | 468 → 459, 398 → 384 |
| Gene . | Primer Name . | Sequence (5′ → 3′) . | Positions . |
|---|---|---|---|
| ICAM-136 | 5I (5′ primer) | GGTGTATGAACTGAGCAATGTGCAAGAAG | 243 → 271 |
| 3I (3′ primer) | AGCACCGTGGTCGTGACCTCAG | 533 → 512 | |
| 3delI (3′ hybrid primer) | GTGACCTCAGTGAGGTTGGCCCGGGGTGC | 521 → 512, 451 → 433 | |
| 5I-PCR (5′ primer) | TAGCCAACCAATGTGCTATTCAAACT | 273 → 298 | |
| VCAM-137 | 5V (5′ primer) | AGATTGGTGACTCCGTCTCATTGACTTG | 113 → 140 |
| 3V (3′ primer) | CTGAACACTTGACTGTGATCGGCTTC | 415 → 390 | |
| 3delV (3′ hybrid primer) | GATCGGCTTCACCTGGATTCCTTTTTCCAA | 399 → 390, 329 → 310 | |
| GAPDH38 | 5G (5′ primer) | GGTTTACATGTTCCAATATGATTCCACCC | 189 → 217 |
| 3G (3′ primer) | GTTGTCATACTTCTCATGGTTCACACCC | 486 → 459 | |
| 3delG (3′ hybrid primer) | GTTCACACCCTGCAAATGAGCCCCA | 468 → 459, 398 → 384 |