Table 1. Primers used for site-directed... | American Society of Hematology

Table 1.

Primers used for site-directed mutagenesis

Primer	Sequence (5′ → 3′)^*	Location Exon/Nucleotide^†
25A	GGG CTC GAG TAT ACC AAA ACG	18/2353-2373
95A	GAA GTG GAA CTT CAC AGA CCA TG	20/2562-2584
91B	TGT CGG GAC CAG AAG TGG AAC	20/2551-2571
NheI-O	AGT TTC CCA GCT TCT TAT TTT GAT G	29/5101-5125

Primer	Sequence (5′ → 3′)^*	Location Exon/Nucleotide^†
25A	GGG CTC GAG TAT ACC AAA ACG	18/2353-2373
95A	GAA GTG GAA CTT CAC AGA CCA TG	20/2562-2584
91B	TGT CGG GAC CAG AAG TGG AAC	20/2551-2571
NheI-O	AGT TTC CCA GCT TCT TAT TTT GAT G	29/5101-5125

Underlined nucleotides correspond to the substitutions.

Nucleotides are numbered starting from A of the initiating Met codon of exon 2.27