Primer sequences for methylation-specific polymerase chain reaction and reverse transcriptase-polymerase chain reaction and probe sequences for Southern blot analysis
| Primer . | Sequence . |
|---|---|
| p15MF | 5′ GCG TTC GTA TTT TGC GGT T 3′ |
| p15MR | 5′ CGT ACA ATA ACC GAA CGA CCG A 3′ |
| p15UF | 5′ TGT GAT GTG TTT GTA TTT TGT GGT T 3′ |
| p15UR | 5′ CCA TAC AAT AAC CAA ACA ACC AA 3′ |
| p15WF | 5′ CGC ACC CTG CGG CCA GA 3′ |
| p15WR | 5′ AGT GGC CGA GCG GCC GG 3′ |
| p16MF | 5′ TTA TTA GAG GGT GGG GCG GAT CGC 3′ |
| p16MR | 5′ GAC CCC GAA CCG CGA CCG TAA 3′ |
| p16UF | 5′ TTA TTA GAG GGT GGG GTG GAT TGT 3′ |
| p16UR | 5′ CAA CCC CAA ACC ACA ACC ATA A 3′ |
| p16WF | 5′ CAG AGG GTG GGG CGG ACC GC 3′ |
| p16WR | 5′ CGG GCC GCG GCC GTG G 3′ |
| mBCRABLF | 5′ ACC ATC GTG GGC GTC CGC AA 3′ |
| MBCRABLF | 5′ GAA GTG TTT CAG AAG CTC CTC C 3′ |
| BCRABLR | 5′ TTG GTT TGG GCT TCA CAC CAT TCC 3′ |
| AF4MLLF | 5′ GAC TAT CGA CAG CAG CAG ACC TT 3′ |
| AF4MLLR | 5′ TCT TCT TCT TTG TGG GTT TG 3′ |
| AML1ETOF | 5′ AGC CAT GAA GAA CCA GG 3′ |
| AML1ETOR | 5′ AGG CTG TAG GAG AAT GG 3′ |
| p15MP | 5′ TAG GC/TG TTT TTT TTT AGA AGT AAT TTA GG 3′ |
| p16MP | 5′ GAG TAG TAT GGA GTT TTC GGT TGA TTG GTT G 3′ |
| BCRABLP | 5′ TGT GAT TAT AGC CTA AGA CCC GGA GCT TTT C 3′ |
| AF4MLLP | 5′ ACT CAG GGT GAT AGC TGT TTC G 3′ |
| AML1ETOP | 5′ GTC TTC ACA TCC ACA GGT GAG TCT 3′ |
| Primer . | Sequence . |
|---|---|
| p15MF | 5′ GCG TTC GTA TTT TGC GGT T 3′ |
| p15MR | 5′ CGT ACA ATA ACC GAA CGA CCG A 3′ |
| p15UF | 5′ TGT GAT GTG TTT GTA TTT TGT GGT T 3′ |
| p15UR | 5′ CCA TAC AAT AAC CAA ACA ACC AA 3′ |
| p15WF | 5′ CGC ACC CTG CGG CCA GA 3′ |
| p15WR | 5′ AGT GGC CGA GCG GCC GG 3′ |
| p16MF | 5′ TTA TTA GAG GGT GGG GCG GAT CGC 3′ |
| p16MR | 5′ GAC CCC GAA CCG CGA CCG TAA 3′ |
| p16UF | 5′ TTA TTA GAG GGT GGG GTG GAT TGT 3′ |
| p16UR | 5′ CAA CCC CAA ACC ACA ACC ATA A 3′ |
| p16WF | 5′ CAG AGG GTG GGG CGG ACC GC 3′ |
| p16WR | 5′ CGG GCC GCG GCC GTG G 3′ |
| mBCRABLF | 5′ ACC ATC GTG GGC GTC CGC AA 3′ |
| MBCRABLF | 5′ GAA GTG TTT CAG AAG CTC CTC C 3′ |
| BCRABLR | 5′ TTG GTT TGG GCT TCA CAC CAT TCC 3′ |
| AF4MLLF | 5′ GAC TAT CGA CAG CAG CAG ACC TT 3′ |
| AF4MLLR | 5′ TCT TCT TCT TTG TGG GTT TG 3′ |
| AML1ETOF | 5′ AGC CAT GAA GAA CCA GG 3′ |
| AML1ETOR | 5′ AGG CTG TAG GAG AAT GG 3′ |
| p15MP | 5′ TAG GC/TG TTT TTT TTT AGA AGT AAT TTA GG 3′ |
| p16MP | 5′ GAG TAG TAT GGA GTT TTC GGT TGA TTG GTT G 3′ |
| BCRABLP | 5′ TGT GAT TAT AGC CTA AGA CCC GGA GCT TTT C 3′ |
| AF4MLLP | 5′ ACT CAG GGT GAT AGC TGT TTC G 3′ |
| AML1ETOP | 5′ GTC TTC ACA TCC ACA GGT GAG TCT 3′ |