Table 1. Primer pairs and PCR conditions... | American Society of Hematology

Table 1.

Primer pairs and PCR conditions for sequencingWAS cDNA

Primer Set	Sequence (5′-3′)	Exons Covered	Fragment Size (bp^*)
W-2	GCTTCGCCAGAGAAGACAAG	1-4	495
W-496c	TCCACTTTGCCCTCTGATTCC
W-452	CTCGTGCAGGAGAAGATACA	4-9	475
W-926c	CCTGGTCCTCAATGAAGTCG
W-906	ACGACTTCATTGAGGACCAG	9-11	497
W-1402c	CTGCAGCGCTGAGCTCTCTG
W-1406	CCACCACCTCAGAGCTCAGA	11-12	303
W-1708c	TGAGTGTGAGGACCAGGCAG

Primer Set	Sequence (5′-3′)	Exons Covered	Fragment Size (bp^*)
W-2	GCTTCGCCAGAGAAGACAAG	1-4	495
W-496c	TCCACTTTGCCCTCTGATTCC
W-452	CTCGTGCAGGAGAAGATACA	4-9	475
W-926c	CCTGGTCCTCAATGAAGTCG
W-906	ACGACTTCATTGAGGACCAG	9-11	497
W-1402c	CTGCAGCGCTGAGCTCTCTG
W-1406	CCACCACCTCAGAGCTCAGA	11-12	303
W-1708c	TGAGTGTGAGGACCAGGCAG

For all reactions, the amplification mix contained 1.5 mmol/L MgCl₂, and the cycling profile was 30 cycles each of 94°C for 30 sec, 57°C for 1 min, and 72°C for 1 min, with a final extension at 72°C for 5 min.

Indicates base pair.

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