Table 2. Detection of IFN activity in... | American Society of Hematology

Table 2.

Detection of IFN activity in the serum of mice injected with CTX

Treatment	Mouse No.	Hours Posttreatment
Treatment	Mouse No.	12	24	48
Saline	1	< 4	< 4	< 4
	2	< 4	< 4	< 4
	3	< 4	< 4	< 4
CTX 83 mg/kg	1	< 4	< 4	32
	2	< 4	< 4	< 4
	3	< 4	16	32
CTX 150 mg/kg	1	< 4	< 4	< 4
	2	< 4	16	24
	3	< 4	32	48
Poly (I:C)	1	256	64	< 4
	2	256	64	< 4
	3	256	64	< 4

Treatment	Mouse No.	Hours Posttreatment
Treatment	Mouse No.	12	24	48
Saline	1	< 4	< 4	< 4
	2	< 4	< 4	< 4
	3	< 4	< 4	< 4
CTX 83 mg/kg	1	< 4	< 4	32
	2	< 4	< 4	< 4
	3	< 4	16	32
CTX 150 mg/kg	1	< 4	< 4	< 4
	2	< 4	16	24
	3	< 4	32	48
Poly (I:C)	1	256	64	< 4
	2	256	64	< 4
	3	256	64	< 4

Six 7-week-old DBA/2 mice were injected intraperitoneally with 2 different doses of CTX (83 and 150 mg/kg body weight), poly (I:C) (0.15 mg/mouse), or saline. After different time intervals, mice were bled and serum samples collected and frozen. For IFN titration, sera were diluted 1:4 in 2% FCS RPMI 1640 to avoid nonspecific toxicity on cell substrates and processed for a standard IFN assay on L929 mouse cell monolayers, as described in “Materials and methods.” Data represent the IFN titer detected in the serum from each individual mouse, expressed in experimental units. One of these units is the equivalent of 4 international IFN units.

View Large

This Feature Is Available To Subscribers Only