Production of IFN-γ and IL-4 in supernatants from cultures of peripheral blood mononuclear cells stimulated with G-CSF in vivo and in vitro
| . | Before G-CSF . | After G-CSF . |
|---|---|---|
| A | ||
| IL-4 | 1.75 ± 0.25 | 7.5 ± 4.54 |
| IFN-γ | 400 ± 100 | 175 ± 75 |
| B | ||
| IL-4 | 1.8 ± 0.5 | 4 ± 0.4 |
| IFN-γ | 350 ± 87 | 110 ± 35 |
| . | Before G-CSF . | After G-CSF . |
|---|---|---|
| A | ||
| IL-4 | 1.75 ± 0.25 | 7.5 ± 4.54 |
| IFN-γ | 400 ± 100 | 175 ± 75 |
| B | ||
| IL-4 | 1.8 ± 0.5 | 4 ± 0.4 |
| IFN-γ | 350 ± 87 | 110 ± 35 |
In part A, samples of lymphocytes were obtained before and after the administration of G-CSF. IL-4 and IFN-γ concentrations were measured after 24-hour PHA stimulation. In part B, samples of lymphocytes obtained from normal donors were cocultured with G-CSF for 24 hours and subsequently stimulated with anti-CD3 mAb for 48 hours. Concentrations (mean ± SEM of IL-4 and IFN-γ concentration in supernatants) were determined using ELISA and expressed as pg/mL. Results represent the summary of 2 experiments for part A and 5 experiments for part B.