Number of progenitor cells and benzidine-positive cells in the light density fraction of the spleens from normal mice and from mice 1 and 3 days after PHZ treatment*
| Cell Source . | Progenitor Cells per 104 Cells . | Benzidine+ Cells (%) . | ||
|---|---|---|---|---|
| BFU-E . | CFU-E . | CFU-Mk . | ||
| Normal spleen | 4 ± 2 | 2 ± 1 | < 0.3 | 1 ± 1 |
| Spleen from anemic mice | ||||
| Day 1 after PHZ | 140 ± 50† | 21 ± 11‡ | 0.8 ± 0.05 | 17 ± 5† |
| Day 3 after spleen | 15 ± 5‡ | 273 ± 39† | < 0.3 | 69 ± 15† |
| Purified spleen cells1-153 | ||||
| GF-starved cells | 5 ± 2 | < 0.3 | < 0.3 | ≪1 |
| TER119+/4A5− cells | < 0.3 | 3 ± 0.8 | < 0.3 | 100† |
| TER119+/4A5+ cells | < 0.3 | < 0.3 | < 0.3 | ≪1 |
| Cell Source . | Progenitor Cells per 104 Cells . | Benzidine+ Cells (%) . | ||
|---|---|---|---|---|
| BFU-E . | CFU-E . | CFU-Mk . | ||
| Normal spleen | 4 ± 2 | 2 ± 1 | < 0.3 | 1 ± 1 |
| Spleen from anemic mice | ||||
| Day 1 after PHZ | 140 ± 50† | 21 ± 11‡ | 0.8 ± 0.05 | 17 ± 5† |
| Day 3 after spleen | 15 ± 5‡ | 273 ± 39† | < 0.3 | 69 ± 15† |
| Purified spleen cells1-153 | ||||
| GF-starved cells | 5 ± 2 | < 0.3 | < 0.3 | ≪1 |
| TER119+/4A5− cells | < 0.3 | 3 ± 0.8 | < 0.3 | 100† |
| TER119+/4A5+ cells | < 0.3 | < 0.3 | < 0.3 | ≪1 |
BFU-E = burst-forming unit erythroid; CFU-E = colony-forming unit-erythroid; CFU-Mk = colony-forming unit-megakaryocyte; GF = growth factor; PHZ = phenylhydrazine.
The progenitor and benzidine-positive cell content of spleen cells purified by GF starvation or physical separation is also presented for comparison. Values are expressed as mean (± SD) of 3 to 5 separate experiments performed in triplicate.
P < .01 with respect to the values observed in normal spleen.
P < .05 with respect to the values observed in normal spleen.
The TER-119+/4A5± cells were isolated by immunomagnetic selection; see Table 3 and Figure 2 for further details.