Table 5. Total cell number and frequency... | American Society of Hematology

Table 5.

Total cell number and frequency of TER-119⁺ and/or 4A5⁺ cells in cultures of purified double-positive TER-119⁺/4A5⁺cells from the spleen of day 1-2 phenylhydrazine-treated mice^4-150

Time and Factor in Culture	Total Cell Number (× 10⁵)	Phenotype (%)
Time and Factor in Culture	Total Cell Number (× 10⁵)	TER119⁺	4A5⁺	TER119⁺/4A5⁺
0 h	2	20 ± 5	3 ± 1	60 ± 10
−Growth factor
24 h	1.4 ± 0.2	27 ± 7	BD	35 ± 5
48 h	0.4 ± 0.2^‡	10 ± 5	BD	10 ± 7^‡
+Erythropoietin
24 h	1.8 ± 0.2	60 ± 7^‡	8 ± 3^4-151	15 ± 5^‡
48 h	1.2 ± 0.4	75 ± 15^‡	6 ± 2	7 ± 2^‡
+Thrombopoietin
24 h	1.7 ± 0.2	24 ± 4	16 ± 4^‡	27 ± 2^4-151
48 h	1.0 ± 0.4^4-151	62 ± 12^‡	11 ± 2^‡	10 ± 3^‡

Time and Factor in Culture	Total Cell Number (× 10⁵)	Phenotype (%)
Time and Factor in Culture	Total Cell Number (× 10⁵)	TER119⁺	4A5⁺	TER119⁺/4A5⁺
0 h	2	20 ± 5	3 ± 1	60 ± 10
−Growth factor
24 h	1.4 ± 0.2	27 ± 7	BD	35 ± 5
48 h	0.4 ± 0.2^‡	10 ± 5	BD	10 ± 7^‡
+Erythropoietin
24 h	1.8 ± 0.2	60 ± 7^‡	8 ± 3^4-151	15 ± 5^‡
48 h	1.2 ± 0.4	75 ± 15^‡	6 ± 2	7 ± 2^‡
+Thrombopoietin
24 h	1.7 ± 0.2	24 ± 4	16 ± 4^‡	27 ± 2^4-151
48 h	1.0 ± 0.4^4-151	62 ± 12^‡	11 ± 2^‡	10 ± 3^‡

F4-150

The results are presented as the mean (± SD) of 7 separate experiments with cells isolated by immunomagnetic beads. Only propidium iodide–negative cells were considered in the analysis. The sum of the frequencies is not 100% because the frequencies of double (TER-119 and 4A5) negative cells are not reported.

F4-151

P < .01 with respect to the input values at 0 hours.

‡

P < .05 with respect to the input values at 0 hours.

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