Effect of FB1 on daunorubicin-induced apoptosis
| . | Previously Determined IC30 Daunorubicin (μg/mL) . | 7-AAD High without FB1, % . | 7-AAD High with FB1, % . | Inhibition, % . |
|---|---|---|---|---|
| GO | 0.03 | 27.4 | 21.7 | 20.8 |
| JD | 0.1 | 39.7 | 24 | 39.5 |
| RF | 0.05 | 38.5 | 31.6 | 17.9 |
| DS | 0.05 | 31.4 | 22 | 29.9 |
| BB | 0.03 | 32 | 6.2 | 80.6 |
| CL | 0.005 | 21.4 | 12.9 | 39.7 |
| AJ | 0.01 | 21.9 | 4.4 | 79.9 |
| . | Previously Determined IC30 Daunorubicin (μg/mL) . | 7-AAD High without FB1, % . | 7-AAD High with FB1, % . | Inhibition, % . |
|---|---|---|---|---|
| GO | 0.03 | 27.4 | 21.7 | 20.8 |
| JD | 0.1 | 39.7 | 24 | 39.5 |
| RF | 0.05 | 38.5 | 31.6 | 17.9 |
| DS | 0.05 | 31.4 | 22 | 29.9 |
| BB | 0.03 | 32 | 6.2 | 80.6 |
| CL | 0.005 | 21.4 | 12.9 | 39.7 |
| AJ | 0.01 | 21.9 | 4.4 | 79.9 |
Patient cells were cultured for 48 hours in suspension culture with 10% FCS to determine the in vitro IC30 for daunorubicin. Assays were then repeated with and without daunorubicin at the previously determined IC30 for each patient, with and without 50 μmol/L FB1. Background values for cells cultured without daunorubicin have been subtracted.