Table 1.

Primers

Primer Nucleotide sequence Localization Position, range
rb10b  GGCTAAATATTTTGATGACCAAGTT  RHD cDNA 1194 to 1217  
re011d  GCAGCCAACTTCCCCTGTG  RHDpromoter  −883 to −901  
re014  GCTCTACCTTGGTCACCTCC dJ469D22  52189 to 52209  
re04  AGGTCACATCCATTTATCCCACTG dJ469D22  53968 to 53945  
re11d AGAAGATGGGGGAATCTTTTTCCT  dJ469D22  51193 to 51216  
re96 TTGTGACTGGGCTAGAAAGAAGGTG  dJ469D22  242 to 216  
rea7 TGTTGCCTGCATTTGTACGTGAG  RHD cDNA  1311 to 1333 
rend31  TTCTGTCTGGGTTGGGGAGGG  dJ465N24 128649 to 128629  
rend32  GGAGGGGTTAATATGGGTGGC dJ465N24  127355 to 127375  
rend8b1 TTTGTCCTGGTTGCCTGTGGTC  dJ465N24  69296 to 69274 
rend8b2  CAAATCCTGTTGACTGGTCTCGG  dJ465N24 68451 to 68473  
rend9a1  AACGGCTCCATCACCCCTAAAG dJ465N24  50008 to 49987  
rend9a2 CCCACTCCTAGATACCAACCCAAG  dJ465N24  49059 to 49083 
rey14a  CTTTATGCACTGCCTCGTTGAATC  dJ469D22 56792 to 56769  
rey14b  TTGACTGGTGTGGTTGCTGTTG  dJ469D22 55863 to 55884  
rey15a  GCAGAAAGGGGAGTTGATGCTG  dJ469D22 55416 to 55395  
rey7  CTGACAAAGTTGAGAGCCCACTG  dJ469D22 62324 to 62346  
rey8  TTAAGCCTACATCCACATGCTGAG  dJ469D22 62854 to 62831  
rez2  CCTTGGTCTGCCAGAATTTTCA RHD cDNA  2738 to 2717  
rez4 GTTTGGCATCATAGGAGATTTGGC  dJ465N24  120101 to 120124 
rez7  CCTGTCCCCATGATTCAGTTACC  dJ465N24 124831 to 124854  
rh7  ACGTACAAATGCAGGCAAC  RHDcDNA  1330 to 1312  
rnb31  CCTTTTTTTGTTTGTTTTTGGCGGTGC downstream Rhesus box 1330 to 1312  
rr4 AGCTTACTGGATGACCACCA  RHD cDNA  1541 to 1522 
sf1  GACTGGGGGGAAAAGCGCAATAC  SMP1 cDNA 142 to 164  
sf1c  GTATTGCGCTTTTCCCCCCAGTC  SMP1cDNA  164 to 142  
sf3  TGACTTGCTCTCATCCCACATG SMP1 cDNA  1696 to 1717  
sm19 GGGCTTGAAGCAAGTAAATGGAAG  SMP1 intron 1 −58 to −35  
sr1  GCTATCAATATTTTCTTGGTTACAGACAC SMP1 cDNA  2172 to 2144  
sr3 GTTCACTGCCATAAGTCTTCAGTGC  SMP1 cDNA  575 to 551 
sr3kp  TGGCCGCACTGAAGACTTATGG  SMP1 cDNA 546 to 567  
sr45  CAGCTGCATCTATGATAATCCACC  SMP1cDNA  224 to 243  
sr47  ATGGACAAGTCCGAGGTGATAG SMP1 cDNA  315 to 344  
sr47c ATCACCTCGGACTTGTCCATTC  SMP1 cDNA  342 to 321 
sr5  GCAATCAGAGATCCAAAGGCCAAC  SMP1 cDNA 428 to 405  
sr5c  GTTGGCCTTTGGATCTCTGATTGC  SMP1cDNA  405 to 428  
sr55  GACATAGTATACCCTGGAATTGCTGT SMP1 cDNA  472 to 497  
sr55c ACAGCAATTCCAGGGTATACTATGTC  SMP1 cDNA  497 to 472 
sr9  CTCCCCCGATTTTAGCCAAGAA  SMP1 cDNA 27 to 6 
Primer Nucleotide sequence Localization Position, range
rb10b  GGCTAAATATTTTGATGACCAAGTT  RHD cDNA 1194 to 1217  
re011d  GCAGCCAACTTCCCCTGTG  RHDpromoter  −883 to −901  
re014  GCTCTACCTTGGTCACCTCC dJ469D22  52189 to 52209  
re04  AGGTCACATCCATTTATCCCACTG dJ469D22  53968 to 53945  
re11d AGAAGATGGGGGAATCTTTTTCCT  dJ469D22  51193 to 51216  
re96 TTGTGACTGGGCTAGAAAGAAGGTG  dJ469D22  242 to 216  
rea7 TGTTGCCTGCATTTGTACGTGAG  RHD cDNA  1311 to 1333 
rend31  TTCTGTCTGGGTTGGGGAGGG  dJ465N24 128649 to 128629  
rend32  GGAGGGGTTAATATGGGTGGC dJ465N24  127355 to 127375  
rend8b1 TTTGTCCTGGTTGCCTGTGGTC  dJ465N24  69296 to 69274 
rend8b2  CAAATCCTGTTGACTGGTCTCGG  dJ465N24 68451 to 68473  
rend9a1  AACGGCTCCATCACCCCTAAAG dJ465N24  50008 to 49987  
rend9a2 CCCACTCCTAGATACCAACCCAAG  dJ465N24  49059 to 49083 
rey14a  CTTTATGCACTGCCTCGTTGAATC  dJ469D22 56792 to 56769  
rey14b  TTGACTGGTGTGGTTGCTGTTG  dJ469D22 55863 to 55884  
rey15a  GCAGAAAGGGGAGTTGATGCTG  dJ469D22 55416 to 55395  
rey7  CTGACAAAGTTGAGAGCCCACTG  dJ469D22 62324 to 62346  
rey8  TTAAGCCTACATCCACATGCTGAG  dJ469D22 62854 to 62831  
rez2  CCTTGGTCTGCCAGAATTTTCA RHD cDNA  2738 to 2717  
rez4 GTTTGGCATCATAGGAGATTTGGC  dJ465N24  120101 to 120124 
rez7  CCTGTCCCCATGATTCAGTTACC  dJ465N24 124831 to 124854  
rh7  ACGTACAAATGCAGGCAAC  RHDcDNA  1330 to 1312  
rnb31  CCTTTTTTTGTTTGTTTTTGGCGGTGC downstream Rhesus box 1330 to 1312  
rr4 AGCTTACTGGATGACCACCA  RHD cDNA  1541 to 1522 
sf1  GACTGGGGGGAAAAGCGCAATAC  SMP1 cDNA 142 to 164  
sf1c  GTATTGCGCTTTTCCCCCCAGTC  SMP1cDNA  164 to 142  
sf3  TGACTTGCTCTCATCCCACATG SMP1 cDNA  1696 to 1717  
sm19 GGGCTTGAAGCAAGTAAATGGAAG  SMP1 intron 1 −58 to −35  
sr1  GCTATCAATATTTTCTTGGTTACAGACAC SMP1 cDNA  2172 to 2144  
sr3 GTTCACTGCCATAAGTCTTCAGTGC  SMP1 cDNA  575 to 551 
sr3kp  TGGCCGCACTGAAGACTTATGG  SMP1 cDNA 546 to 567  
sr45  CAGCTGCATCTATGATAATCCACC  SMP1cDNA  224 to 243  
sr47  ATGGACAAGTCCGAGGTGATAG SMP1 cDNA  315 to 344  
sr47c ATCACCTCGGACTTGTCCATTC  SMP1 cDNA  342 to 321 
sr5  GCAATCAGAGATCCAAAGGCCAAC  SMP1 cDNA 428 to 405  
sr5c  GTTGGCCTTTGGATCTCTGATTGC  SMP1cDNA  405 to 428  
sr55  GACATAGTATACCCTGGAATTGCTGT SMP1 cDNA  472 to 497  
sr55c ACAGCAATTCCAGGGTATACTATGTC  SMP1 cDNA  497 to 472 
sr9  CTCCCCCGATTTTAGCCAAGAA  SMP1 cDNA 27 to 6 

For the RHD promoter and the RHD cDNA, the positions refer to the distance from A of the start codon. For introns, they refer to the distance from the intron/exon junction. For all other sequences, including SMP1 cDNA, they refer to the distance from the start of the published sequences. The mismatches in primers rey14b, rnb31, and sf3 were inadvertently introduced. Primers re11d, re014, and re04 do not exactly match dJ469D22 because they were designed from our raw sequences covering the 5′ flanking region of RHD.

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