Table 2. The presence of RHD flanking... | American Society of Hematology

Table 2.

The presence of RHD flanking sequences in YAC 38A-A10

Primer		Predicted position	Amplicon size, bp	Amplicons obtained?
Primer				Genomic DNA		YAC 38A-A10
Sense	Antisense			RHD⁺	RHD⁻	YAC 38A-A10
rend9a1	rend9a2	RHD 5′ flanking region about 85 000 bp from ATG	948	yes	yes	yes
rend8b1	rend8b2	RHD 5′ flanking region about 50 000 bp from ATG	845	yes	yes	yes
rea7	rez2	RHD3′ flanking region about 1500 bp from STOP	1412	yes	no	yes
rend32	sr9	RHCE 3′ flanking region about 20 000 bp from STOP	1989	yes	yes	yes
sr1	sf3	RHCE 3′ flanking region about 1000 bp from STOP	477	yes	yes	yes
rey14b	rey14a	RHCE 5′ flanking region about 5300 bp from ATG	929	yes	yes	no
rey7	rey8	RHCE 5′ flanking region about 10 000 bp from ATG	530	yes	yes	no

Primer		Predicted position	Amplicon size, bp	Amplicons obtained?
Primer				Genomic DNA		YAC 38A-A10
Sense	Antisense			RHD⁺	RHD⁻	YAC 38A-A10
rend9a1	rend9a2	RHD 5′ flanking region about 85 000 bp from ATG	948	yes	yes	yes
rend8b1	rend8b2	RHD 5′ flanking region about 50 000 bp from ATG	845	yes	yes	yes
rea7	rez2	RHD3′ flanking region about 1500 bp from STOP	1412	yes	no	yes
rend32	sr9	RHCE 3′ flanking region about 20 000 bp from STOP	1989	yes	yes	yes
sr1	sf3	RHCE 3′ flanking region about 1000 bp from STOP	477	yes	yes	yes
rey14b	rey14a	RHCE 5′ flanking region about 5300 bp from ATG	929	yes	yes	no
rey7	rey8	RHCE 5′ flanking region about 10 000 bp from ATG	530	yes	yes	no