Primers used for PCR amplifications (5′ >> 3′)
| Target . | Forward . | Reverse . |
|---|---|---|
| Exon 1 | CAGCCCCACCCTTAGAAAAG | CCTGGGGTCATAAAGCTAAG |
| Exons 2-3 | CCTCTTCTGGCTAACATTGC | CAGGGATATTATGAAGGTATG |
| Exon 4 | CAGCAGCTACTTCAATAACC | GTGCATAACTATCGCCTTCC |
| Exon 5, 5′ | (same as exon 4) | GCGGCATGTCTGTTAATGCC |
| Exon 5, mid | GATCTTGTCGAGGGTCATGC | CTCCTAACATAGGTGAGAAG |
| Exon 5, 3′ | CTGGACCTCTGAGAGCTCTG | ATGGCTCTGTACTGTTAGGC |
| Target . | Forward . | Reverse . |
|---|---|---|
| Exon 1 | CAGCCCCACCCTTAGAAAAG | CCTGGGGTCATAAAGCTAAG |
| Exons 2-3 | CCTCTTCTGGCTAACATTGC | CAGGGATATTATGAAGGTATG |
| Exon 4 | CAGCAGCTACTTCAATAACC | GTGCATAACTATCGCCTTCC |
| Exon 5, 5′ | (same as exon 4) | GCGGCATGTCTGTTAATGCC |
| Exon 5, mid | GATCTTGTCGAGGGTCATGC | CTCCTAACATAGGTGAGAAG |
| Exon 5, 3′ | CTGGACCTCTGAGAGCTCTG | ATGGCTCTGTACTGTTAGGC |