BPI induces nuclear fragmentation in HUVECs
| Condition . | Percentage of cells with fragmented nuclei . | |
|---|---|---|
| Experiment 1 . | Experiment 2 . | |
| Control | 5* | 3 |
| BPI (1.8 μmol/L) | 79 | 69 |
| PF4 (1.8 μmol/L) | 7 | 4 |
| BSA (1.8 μmol/L) | 4 | 5 |
| 1% HS | 99 | 71 |
| Condition . | Percentage of cells with fragmented nuclei . | |
|---|---|---|
| Experiment 1 . | Experiment 2 . | |
| Control | 5* | 3 |
| BPI (1.8 μmol/L) | 79 | 69 |
| PF4 (1.8 μmol/L) | 7 | 4 |
| BSA (1.8 μmol/L) | 4 | 5 |
| 1% HS | 99 | 71 |
HUVECs were cultured for 3 days under indicated conditions, collected, cytospinned, and stained with haematoxillin/eosin. Percentage of fragmented nuclei was microscopically scored. BPI, bactericidal/permeability-increasing protein; HUVEC, human umbilical vein-derived endothelial cell; PF4, platelet factor-4; BSA, bovine serum albumin; HS, human pooled serum.