Ratio of colony numbers showing identical nucleotide sequences per picked-up colonies
| Case . | IGH . | TCRD . | ||
|---|---|---|---|---|
| Guthrie card . | Leukemic cell . | Guthrie card . | Leukemic cell . | |
| 1 | 14/15 (93.3) | 15/16 (93.8) | 13/15 (86.7) | 12/15 (80.0) |
| 2 | 11/17 (64.7) | 13/15 (86.7) | NA | NA |
| 3 | 13/18 (72.2) | 15/21 (71.4) | 8/19 (42.1) | 11/16 (68.8) |
| 4a | 14/21 (66.7) | 13/16 (81.3) | 6/15 (40.0) | 10/18 (55.6) |
| 4b | 9/16 (56.3) | NA | ||
| 5 | NA | 12/16 (75.0) | NA | NA |
| 6 | NA | 8/15 (53.3) | NA | 10/17 (58.8) |
| 7 | NA | 13/16 (81.3) | NA | 12/18 (66.7) |
| Case . | IGH . | TCRD . | ||
|---|---|---|---|---|
| Guthrie card . | Leukemic cell . | Guthrie card . | Leukemic cell . | |
| 1 | 14/15 (93.3) | 15/16 (93.8) | 13/15 (86.7) | 12/15 (80.0) |
| 2 | 11/17 (64.7) | 13/15 (86.7) | NA | NA |
| 3 | 13/18 (72.2) | 15/21 (71.4) | 8/19 (42.1) | 11/16 (68.8) |
| 4a | 14/21 (66.7) | 13/16 (81.3) | 6/15 (40.0) | 10/18 (55.6) |
| 4b | 9/16 (56.3) | NA | ||
| 5 | NA | 12/16 (75.0) | NA | NA |
| 6 | NA | 8/15 (53.3) | NA | 10/17 (58.8) |
| 7 | NA | 13/16 (81.3) | NA | 12/18 (66.7) |
NA indicates not amplified; parentheses contain percentages; IGH indicates immunoglobulin heavy chain; TCRD, T-cell receptor delta. The rearranged IGH and TCRD regions were amplified with the use of consensus primer pairs (see text). The nucleotide sequences determined are shown in Table 3 and 4.