Comparison of nucleotide sequences in rearranged IGH CDR3 leukemic cell DNA (L) and corresponding Guthrie card DNA (G)3-150
| Case . | V . | N . | D . | N . | J . | DH . | JH . |
|---|---|---|---|---|---|---|---|
| 1(G) | TGTGCAAGAGATCCC | c | TAGTAGTACCAGCT | cgtcc | TTACTACTACTACATGGAC | Dlr-4b | J6 |
| 1(L) | TGTGCAAGAGATCCC | c | TAGTAGTACCAGCT | cgtcc | TTACTACTACTACATGGAC | ||
| 2(G) | TGTGCAAGAGATCC | GTATAGCAGCTCGTCC | ccaa | TACTACTACATGGAC | DN4 | J6 | |
| 2(L) | TGTGCAAGAGATCC | GTATAGCAGCTCGTCC | ccaa | TACTACTACATGGAC | |||
| 3(G) | CAGGGGTCCGTATTTT | TATGGTTCG | ccg | CTGGTTCGACCCCTGG | Dxp′-1 | J5 | |
| 3(L) | CAGGGGTCCGTATTTT | TATGGTTCG | ccg | CTGGTTCGACCCCTGG | |||
| 4a(G)3-151 | TGTGCAAGAGATCCC | aaggat | TAGCAGCTCG | cccct | TTACTACTACATGGAC | DN4 | J6 |
| 4a(L) | TGTGCAAGAGATCCC | aaggat | TAGCAGCTCG | cccct | TTACTACTACATGGAC | ||
| 4b(G) | TGTGCGAGAGATC | ATTACGATATTTTGACTGGTTATTA | ACTGGTTCGACCCCTG | Dxp-1 | J5 | ||
| 4b(L) | not amplified | ||||||
| 5(G) | not amplified | ||||||
| 5(L) | TGTGCAAGAGA TCCC | cct | TATTACTATGGTTCGGGGAGTTATTATTAC | gttatta | CTACTTTGACTACTGG | Dxp′-1 | J4 |
| 6(G) | not amplified | ||||||
| 6(L) | TGTGCAAGAGATCCC | ccggattc | AGCAGCTCGTCC | cc | TTACTACTACTATGGAC | DN4 | J6 |
| 7(G) | not amplified | ||||||
| 7(L) | TGTGCGAGATC | tgg | TAGTACTACTTT | g | TTGACTACTGGGGCCAA | Dlr-5 | J6 |
| Case . | V . | N . | D . | N . | J . | DH . | JH . |
|---|---|---|---|---|---|---|---|
| 1(G) | TGTGCAAGAGATCCC | c | TAGTAGTACCAGCT | cgtcc | TTACTACTACTACATGGAC | Dlr-4b | J6 |
| 1(L) | TGTGCAAGAGATCCC | c | TAGTAGTACCAGCT | cgtcc | TTACTACTACTACATGGAC | ||
| 2(G) | TGTGCAAGAGATCC | GTATAGCAGCTCGTCC | ccaa | TACTACTACATGGAC | DN4 | J6 | |
| 2(L) | TGTGCAAGAGATCC | GTATAGCAGCTCGTCC | ccaa | TACTACTACATGGAC | |||
| 3(G) | CAGGGGTCCGTATTTT | TATGGTTCG | ccg | CTGGTTCGACCCCTGG | Dxp′-1 | J5 | |
| 3(L) | CAGGGGTCCGTATTTT | TATGGTTCG | ccg | CTGGTTCGACCCCTGG | |||
| 4a(G)3-151 | TGTGCAAGAGATCCC | aaggat | TAGCAGCTCG | cccct | TTACTACTACATGGAC | DN4 | J6 |
| 4a(L) | TGTGCAAGAGATCCC | aaggat | TAGCAGCTCG | cccct | TTACTACTACATGGAC | ||
| 4b(G) | TGTGCGAGAGATC | ATTACGATATTTTGACTGGTTATTA | ACTGGTTCGACCCCTG | Dxp-1 | J5 | ||
| 4b(L) | not amplified | ||||||
| 5(G) | not amplified | ||||||
| 5(L) | TGTGCAAGAGA TCCC | cct | TATTACTATGGTTCGGGGAGTTATTATTAC | gttatta | CTACTTTGACTACTGG | Dxp′-1 | J4 |
| 6(G) | not amplified | ||||||
| 6(L) | TGTGCAAGAGATCCC | ccggattc | AGCAGCTCGTCC | cc | TTACTACTACTATGGAC | DN4 | J6 |
| 7(G) | not amplified | ||||||
| 7(L) | TGTGCGAGATC | tgg | TAGTACTACTTT | g | TTGACTACTGGGGCCAA | Dlr-5 | J6 |