Table 2. Day-to-day and multicenter... | American Society of Hematology

Table 2.

Day-to-day and multicenter reproducibility of quantitating malignant myeloma cells using real-time IgH ASO-PCR or a limiting dilution assay

Patient	Center	Quantitation method	No.analyses	Type of variation	Input tumor cells (no.)	Calculated tumor cells (no.)	ASO-PCR variation	Control PCR variation	Total variation	Average per patient	Average total
								Variation coefficient
6	E	Limiting dilution	3	Day-to-day	9.6	10.1	0.24	0.56	0.61
6	C	Limiting dilution	3	Day-to-day	9.6	13.7	0.32	0.92	0.97
6	A	Real time PCR	4	Day-to-day	9.6	12.6	0.15	0.26	0.30
6	E, C, G, U	Limiting dilution	Single	Multicenter (4)	9.6	11	0.24	0.72	0.76
6	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	9.6	10.7	0.11	0.44	0.45
6	E	Limiting dilution	3	Day-to-day	1600	2306	0.09	1.04	1.04	0.83
6	C	Limiting dilution	3	Day-to-day	1600	695	0.65	0.84	1.06	1.02
6	A	Real-time PCR	4	Day-to-day	1600	1890	0.32	0.14	0.35	0.33
6	E, C, G, U	Limiting dilution	Single	Multicenter (4)	1608	1287	0.50	0.89	1.02	0.89
6	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	1608	1701	0.45	0.49	0.67	0.56
H	E	Limiting dilution	3	Day-to-day	56	54	0.64	0.49	0.81
H	C	Limiting dilution	3	Day-to-day	56	26	0.28	0.22	0.36
H	A	Real-time PCR	4	Day-to-day	56	33	0.46	0.14	0.48
H	E, C, G, U	Limiting dilution	Single	Multicenter (4)	56	45	0.73	0.50	0.89
H	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	56	45	0.55	0.37	0.66
H	E	Limiting dilution	3	Day-to-day	560	866	0.40	0.33	0.52	0.67	0.75
H	C	Limiting dilution	3	Day-to-day	560	956	0.56	0.04	0.56	0.46	0.74
H	A	Real-time PCR	4	Day-to-day	560	1702	0.27	0.10	0.29	0.39	0.36
H	E, C, G, U	Limiting dilution	Single	Multicenter (4)	560	993	0.43	0.44	0.62	0.76	0.83
H	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	560	990	0.34	0.44	0.56	0.61	0.59

Patient	Center	Quantitation method	No.analyses	Type of variation	Input tumor cells (no.)	Calculated tumor cells (no.)	ASO-PCR variation	Control PCR variation	Total variation	Average per patient	Average total
								Variation coefficient
6	E	Limiting dilution	3	Day-to-day	9.6	10.1	0.24	0.56	0.61
6	C	Limiting dilution	3	Day-to-day	9.6	13.7	0.32	0.92	0.97
6	A	Real time PCR	4	Day-to-day	9.6	12.6	0.15	0.26	0.30
6	E, C, G, U	Limiting dilution	Single	Multicenter (4)	9.6	11	0.24	0.72	0.76
6	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	9.6	10.7	0.11	0.44	0.45
6	E	Limiting dilution	3	Day-to-day	1600	2306	0.09	1.04	1.04	0.83
6	C	Limiting dilution	3	Day-to-day	1600	695	0.65	0.84	1.06	1.02
6	A	Real-time PCR	4	Day-to-day	1600	1890	0.32	0.14	0.35	0.33
6	E, C, G, U	Limiting dilution	Single	Multicenter (4)	1608	1287	0.50	0.89	1.02	0.89
6	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	1608	1701	0.45	0.49	0.67	0.56
H	E	Limiting dilution	3	Day-to-day	56	54	0.64	0.49	0.81
H	C	Limiting dilution	3	Day-to-day	56	26	0.28	0.22	0.36
H	A	Real-time PCR	4	Day-to-day	56	33	0.46	0.14	0.48
H	E, C, G, U	Limiting dilution	Single	Multicenter (4)	56	45	0.73	0.50	0.89
H	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	56	45	0.55	0.37	0.66
H	E	Limiting dilution	3	Day-to-day	560	866	0.40	0.33	0.52	0.67	0.75
H	C	Limiting dilution	3	Day-to-day	560	956	0.56	0.04	0.56	0.46	0.74
H	A	Real-time PCR	4	Day-to-day	560	1702	0.27	0.10	0.29	0.39	0.36
H	E, C, G, U	Limiting dilution	Single	Multicenter (4)	560	993	0.43	0.44	0.62	0.76	0.83
H	E, C, G, U	Limiting dilution	Triplo 5	Multicenter (4)	560	990	0.34	0.44	0.56	0.61	0.59

The factor-range expresses the quotient of actual maximal and minimal values found in the quantitation assays.

Triplo 5, mean of 5 PCR reactions; whole procedure is repeated 3 times (15 PCR).