Table 3.

Quantitation reproducibility using real-time ASO-PCR or conventional ASO-PCR and densitometric scanning

Patient Quantitation method ProbeType of variationInput tumor cellsCalculated quantityVariation coefficientQuantity interval (95%) Average variations Variation coefficient
H  Real-time PCR  Fr2  Intra PCR  1425 1422  0.2  1141-1703  
   Day-to-day  1425 1634  0.23  1252-2016  
   Intra PCR  1.4 0.8  0.14  0.7-0.9  Real-time PCR intra PCR  0.17 
   Day-to-day  1.4  1.7  0.41  1.0-2.4 Real-time PCR inter PCR  0.32  
H  Real-time PCR  Fr3 Intra PCR  1425  2026  0.1  1830-2222 
   Day-to-day  1425  1493  0.36  958-2028 
   Intra PCR  1.4  1.2  0.37  0.8-1.6 Real-time PCR intra PCR  0.24  
   Day-to-day  1.4 1.6  0.47  0.8-2.4  Real-time PCR inter PCR  0.42  
H Real-time PCR  Fr2 and Fr3       Real-time PCR intra PCR  0.21  
        Real-time PCR inter PCR  0.37  
6  Real-time PCR  Fr2  Intra PCR 2250  2601  0.04  2491-2711  
   Day-to-day 2250  1677  0.57  715-2639  
   Intra PCR 22  21.3  0.36  13.7-28.9  Real-time PCR intra PCR 0.2  
   Day-to-day  22  18.4  0.37 11.6-25.2  Real-time PCR inter PCR  0.47  
6  Real-time PCR  Fr3  Intra PCR  2250  3543  0.17  2990-4136 
   Day-to-day  2250  3010  0.34  1994-4026 
   Intra PCR  22  18  0.24  13.7-22.2 Real-time PCR intra PCR  0.21  
   Day-to-day  22 18.7  0.28  13.5-23.9  Real-time PCR inter PCR  0.31 
6  Real-time PCR  Fr2 and Fr3      Real-time PCR intra PCR  0.21 
        Real-time PCR inter PCR  0.39 
H  Densitometric  Fr2  Intra PCR  1425  5065 0.68  1609-8521  
   Intra PCR  1.4  6.5 0.72  1.8-11.2  Densitometric patient H  0.7  
6 Densitometric  Fr2  Intra PCR  2250  11004  0.88 1318-20690  
   Intra PCR  22  16.5  0.78 3.6-29.4  Densitometric patient 6  0.83  
All        Densitometric  0.77 
        Real-time PCR intra PCR  0.21 
        Real-time PCR inter PCR 0.38 
Patient Quantitation method ProbeType of variationInput tumor cellsCalculated quantityVariation coefficientQuantity interval (95%) Average variations Variation coefficient
H  Real-time PCR  Fr2  Intra PCR  1425 1422  0.2  1141-1703  
   Day-to-day  1425 1634  0.23  1252-2016  
   Intra PCR  1.4 0.8  0.14  0.7-0.9  Real-time PCR intra PCR  0.17 
   Day-to-day  1.4  1.7  0.41  1.0-2.4 Real-time PCR inter PCR  0.32  
H  Real-time PCR  Fr3 Intra PCR  1425  2026  0.1  1830-2222 
   Day-to-day  1425  1493  0.36  958-2028 
   Intra PCR  1.4  1.2  0.37  0.8-1.6 Real-time PCR intra PCR  0.24  
   Day-to-day  1.4 1.6  0.47  0.8-2.4  Real-time PCR inter PCR  0.42  
H Real-time PCR  Fr2 and Fr3       Real-time PCR intra PCR  0.21  
        Real-time PCR inter PCR  0.37  
6  Real-time PCR  Fr2  Intra PCR 2250  2601  0.04  2491-2711  
   Day-to-day 2250  1677  0.57  715-2639  
   Intra PCR 22  21.3  0.36  13.7-28.9  Real-time PCR intra PCR 0.2  
   Day-to-day  22  18.4  0.37 11.6-25.2  Real-time PCR inter PCR  0.47  
6  Real-time PCR  Fr3  Intra PCR  2250  3543  0.17  2990-4136 
   Day-to-day  2250  3010  0.34  1994-4026 
   Intra PCR  22  18  0.24  13.7-22.2 Real-time PCR intra PCR  0.21  
   Day-to-day  22 18.7  0.28  13.5-23.9  Real-time PCR inter PCR  0.31 
6  Real-time PCR  Fr2 and Fr3      Real-time PCR intra PCR  0.21 
        Real-time PCR inter PCR  0.39 
H  Densitometric  Fr2  Intra PCR  1425  5065 0.68  1609-8521  
   Intra PCR  1.4  6.5 0.72  1.8-11.2  Densitometric patient H  0.7  
6 Densitometric  Fr2  Intra PCR  2250  11004  0.88 1318-20690  
   Intra PCR  22  16.5  0.78 3.6-29.4  Densitometric patient 6  0.83  
All        Densitometric  0.77 
        Real-time PCR intra PCR  0.21 
        Real-time PCR inter PCR 0.38 

The factor-range expresses the quotient of actual maximal and minimal values found in the quantitation assays.

View Large
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal