A comparison of XY FISH and BCR-ABL RT-PCR in 11 CML patients after sex-mismatched BMT

Patient no. Months after BMT % host by XY FISH (no. of cells) % BCR-ABL positive by FISH (no. of cells) RT-PCR BCR-ABL/ABL ratio, %
1  15 0.3 (1000)    1 (200)  1.0   
2  28 0.2 (1000)  ND  0.03  
3  19 0.2 (515)  ND  * 
4  7.5   0 (500)  ND  * 
5  33    0 (500) ND  * 
6  9    0 (500) 0.5 (200)  * 
7  18    0 (500)   0 (200)  * 
8  182    0 (500)  ND * 
9  2    0 (500)  ND  * 
10 7    0 (1000)  ND  * 
11  72   0 (503)  ND  * 
Patient no. Months after BMT % host by XY FISH (no. of cells) % BCR-ABL positive by FISH (no. of cells) RT-PCR BCR-ABL/ABL ratio, %
1  15 0.3 (1000)    1 (200)  1.0   
2  28 0.2 (1000)  ND  0.03  
3  19 0.2 (515)  ND  * 
4  7.5   0 (500)  ND  * 
5  33    0 (500) ND  * 
6  9    0 (500) 0.5 (200)  * 
7  18    0 (500)   0 (200)  * 
8  182    0 (500)  ND * 
9  2    0 (500)  ND  * 
10 7    0 (1000)  ND  * 
11  72   0 (503)  ND  * 
*

Negative by nested RT-PCR, not quantified.

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