In the absence of TCR-stimulation, T-HA cells were cultured in the presence of IL-2, IL-15, or without cytokine. Blocking concentrations of anti-IL-2Rα (50 μg/mL), anti-IL-2/15Rβ (20 μg/mL), and anti-γ_c (20 μg/mL) were added to the cultures where indicated. After 48 hours, T-HA cells were harvested and cell death was measured by PI uptake and flow cytometry. Results are expressed as percent survival and represent 2 pooled wells. This experiment was repeated twice. IL indicates interleukin.