Analysis of -globin expression in transgenic mice carrying the cDH2 construct
| Line . | Approximate copy number . | Expression in embryos, % ([ζH + αH]/[ζM + αM]) . | Expression in adults, % (αH/αM) . |
|---|---|---|---|
| α82 | 40 | 44.3 (41.9-51.4) | —* |
| α83 | 100 | 30.2 (20.3-40.4) | 8.3 (3.4-14.9) |
| α84 | 20 | 67 (64.5-71.8) | 13.4 (5.6-29.2) |
| α86L | 10 | 36.3 (14.2-47.8) | 16.7 (4.7-39.4) |
| α86H | 20 | 41.4 (32.0-47.8) | 3.4 (1.4-7.0) |
| Line . | Approximate copy number . | Expression in embryos, % ([ζH + αH]/[ζM + αM]) . | Expression in adults, % (αH/αM) . |
|---|---|---|---|
| α82 | 40 | 44.3 (41.9-51.4) | —* |
| α83 | 100 | 30.2 (20.3-40.4) | 8.3 (3.4-14.9) |
| α84 | 20 | 67 (64.5-71.8) | 13.4 (5.6-29.2) |
| α86L | 10 | 36.3 (14.2-47.8) | 16.7 (4.7-39.4) |
| α86H | 20 | 41.4 (32.0-47.8) | 3.4 (1.4-7.0) |
A full comparison of these data with all other transgenic constructs containing HS-40 and the α-globin genes is given in Higgs et al.14 For example, transgenes containing a 70-kb DNA segment of the human α cluster spanning approximate coordinates 100 000-170 000 expressed from 13%-50% human α-globin mRNA in embryos (compared to mouse α mRNAs) and 3%-30% in adults.55
Indicates that these mice died as embryos.