The role of donor or recipient CD4+ cells on elimination responses
| Donor . | Recipient . | % donor cells (mean ± SD) . | No. of mice . | |
|---|---|---|---|---|
| Spleen . | Lymph nodes . | |||
| DBA/2 | C57BL/6 | 0.04 ± 0.03* | 0.10 ± 0.12* | 6 |
| DBA/2 | CD4 KO | 0.03 ± 0.01* | 0.03 ± 0.05* | 3 |
| DBA/2 | MHC II KO | 4.00 ± 0.58† | 3.30 ± 0.46*† | 3 |
| C57BL/6 | C57BL/6 | 5.82 ± 1.47† | 6.87 ± 1.70† | 6 |
| C57BL/6 | DBA/2 | 0.23 ± 0.09* | 0.39 ± 0.29* | 5 |
| CD4 KO | DBA/2 | 1.73 ± 0.70*† | 4.10 ± 0.86*† | 3 |
| MHC II KO | DBA/2 | 4.86 ± 1.23† | 7.39 ± 1.56† | 3 |
| DBA/2 | DBA/2 | 5.85 ± 1.69† | 6.74 ± 0.84† | 5 |
| Donor . | Recipient . | % donor cells (mean ± SD) . | No. of mice . | |
|---|---|---|---|---|
| Spleen . | Lymph nodes . | |||
| DBA/2 | C57BL/6 | 0.04 ± 0.03* | 0.10 ± 0.12* | 6 |
| DBA/2 | CD4 KO | 0.03 ± 0.01* | 0.03 ± 0.05* | 3 |
| DBA/2 | MHC II KO | 4.00 ± 0.58† | 3.30 ± 0.46*† | 3 |
| C57BL/6 | C57BL/6 | 5.82 ± 1.47† | 6.87 ± 1.70† | 6 |
| C57BL/6 | DBA/2 | 0.23 ± 0.09* | 0.39 ± 0.29* | 5 |
| CD4 KO | DBA/2 | 1.73 ± 0.70*† | 4.10 ± 0.86*† | 3 |
| MHC II KO | DBA/2 | 4.86 ± 1.23† | 7.39 ± 1.56† | 3 |
| DBA/2 | DBA/2 | 5.85 ± 1.69† | 6.74 ± 0.84† | 5 |
FITC-labeled splenocytes from the indicated donor mice were injected intravenously into the indicated recipient mice (1 spleen equivalent per recipient). On day 3, the spleen and lymph nodes were obtained from the recipient mice and the number of FITC-labeled donor cells determined by flow cytometric analysis. Results that are significantly lower (P < .05) than the syngeneic controls are indicated by an asterisk (*), whereas results that are significantly higher (P < .05) than the results obtained with the fully allogeneic control combination (C57BL/6 into DBA/2 or DBA/2 into C57BL/6) are indicated using an dagger (
).