Table 2. Ig light chain gene... | American Society of Hematology

Table 2.

Ig light chain gene configuration in MM patients based on Southern blotting, heteroduplex PCR analysis, and sequencing

Patients	MM-1	MM-2	MM-3	MM-4	MM-5	MM-6	MM-7	MM-8	MM-9	MM-10	MM-11	MM-12
MM phenotype	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igλ⁺	True nonproducer	True nonproducer	True nonproducer
Tumor load	35%	25%	20%	90%	80%	80%	30%	80%	60%	25%	70%	35%
IGK: Southern blot analysis^*
First allele	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Kde	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ + intron-Kde
Second allele	G	Vκ-Kde	Vκ-Jκ + intron-Kde	Vκ-Jκ + intron-Kde	G	G	G	G	Vκ-Jκ + intron-Kde	G	Vκ-Kde	Vκ-Jκ + intron-Kde
IGK:PCR
Gene segments	Vκ4-1/ Jκ2	Vκ3-20/ Jκ4	ND	Vκ1-12/Jκ4 Vκ2D-29/Jκ4	Vκ1-5/ Jκ2	Vκ3-20/ Jκ4	Vκ1-33/ Jκ2	Vκ1-5/ Jκ1	Vκ1D-12/ Jκ4	Negative	Vκ1-39/Jκ2	Vκ1D-43/Jκ5
Reading frame	+	+	ND	+/−	+	+	+	+	−	NA	+	−
Somatic hypermutation	+	+	ND	+/−	+	+	+	+	−	NA	+	−
IGL: Southern blot analysis
Jλ-Cλ regions	G	G	G	G	G	G	G	G	Vλ-Jλ₁/Vλ-Jλ₂	G	G	Vλ-Jλ₁/Vλ-Jλ₂

Patients	MM-1	MM-2	MM-3	MM-4	MM-5	MM-6	MM-7	MM-8	MM-9	MM-10	MM-11	MM-12
MM phenotype	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igκ⁺	Igλ⁺	True nonproducer	True nonproducer	True nonproducer
Tumor load	35%	25%	20%	90%	80%	80%	30%	80%	60%	25%	70%	35%
IGK: Southern blot analysis^*
First allele	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ	Vκ-Kde	Vκ-Jκ	Vκ-Jκ	Vκ-Jκ + intron-Kde
Second allele	G	Vκ-Kde	Vκ-Jκ + intron-Kde	Vκ-Jκ + intron-Kde	G	G	G	G	Vκ-Jκ + intron-Kde	G	Vκ-Kde	Vκ-Jκ + intron-Kde
IGK:PCR
Gene segments	Vκ4-1/ Jκ2	Vκ3-20/ Jκ4	ND	Vκ1-12/Jκ4 Vκ2D-29/Jκ4	Vκ1-5/ Jκ2	Vκ3-20/ Jκ4	Vκ1-33/ Jκ2	Vκ1-5/ Jκ1	Vκ1D-12/ Jκ4	Negative	Vκ1-39/Jκ2	Vκ1D-43/Jκ5
Reading frame	+	+	ND	+/−	+	+	+	+	−	NA	+	−
Somatic hypermutation	+	+	ND	+/−	+	+	+	+	−	NA	+	−
IGL: Southern blot analysis
Jλ-Cλ regions	G	G	G	G	G	G	G	G	Vλ-Jλ₁/Vλ-Jλ₂	G	G	Vλ-Jλ₁/Vλ-Jλ₂

The presence of a Vκ-Jκ rearrangement was assumed from the finding of a rearranged band with the IGKJ5 probe. Rearranged bands found with the IGKDE probe were interpreted as Vκ-Kde joining, if accompanied by deletion of Jκ and Cκ segments, and as intron-Kde rearrangements, if they were found on the same restriction fragment as Vκ-Jκ rearrangements (IGKJ5 probe) together with a Cκ gene deletion (IGKC probe).

ND indicates not determined; NA, not applicable.

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