Detection of vIL-6 in the culture supernatants of cell lines
| Cell line . | Culture without TPA (pg/mL) . | Culture with TPA (pg/mL) . |
|---|---|---|
| BC-1*,† | 2880 | 26 300 |
| BCP-1* | 19 200 | 118 100 |
| BCBL-1* | 1680 | 29 100 |
| Daudi† | <30 | <30 |
| v6O‡ | 88 600 | ND |
| VDS-O1†,1-153 | <30 | ND |
| Cell line . | Culture without TPA (pg/mL) . | Culture with TPA (pg/mL) . |
|---|---|---|
| BC-1*,† | 2880 | 26 300 |
| BCP-1* | 19 200 | 118 100 |
| BCBL-1* | 1680 | 29 100 |
| Daudi† | <30 | <30 |
| v6O‡ | 88 600 | ND |
| VDS-O1†,1-153 | <30 | ND |
To prepare conditioned media, suspension cells were seeded in 12-well plates at 1 × 106 cells/well in 2.5 mL RPMI 1640 medium supplemented with 10% fetal bovine serum and cultured with or without 20 ng/mL TPA (Sigma) for 48 hours. Adherent cells were seeded in 6-well plates at 1 × 106 cells/well in 2.5 mL culture media and cultured for 48 hours. The concentration of fetal bovine serum in each supernatant was adjusted to be 10%. Data represent the mean of duplicate assays.
KSHV-positive cell lines.4,20 22
EBV-positive cell lines.12
NIH3T3 cells stably transfected with vIL-6 expression vector.11
Lymphoblastoid cells stably transfected with hIL-6-expression vector.21
ND, not determined.