Anti-HTLV-I response of CD8+ T cells that does not require direct cell-to-cell contact
| Carrier . | p19 Ag level in culture supernatant (pg/mL) . | ||
|---|---|---|---|
| CD8−PBMCs . | CD8− PBMCs plus: . | ||
| auto CD8+ (trans-well) . | auto CD8+ (coculture) . | ||
| 1 | 1280 | 100 (92%) | 71 (95%) |
| 2 | 4080 | 2200 (46%) | 360 (91%) |
| 3 | 4930 | 2020 (59%) | 1110 (79%) |
| Carrier . | p19 Ag level in culture supernatant (pg/mL) . | ||
|---|---|---|---|
| CD8−PBMCs . | CD8− PBMCs plus: . | ||
| auto CD8+ (trans-well) . | auto CD8+ (coculture) . | ||
| 1 | 1280 | 100 (92%) | 71 (95%) |
| 2 | 4080 | 2200 (46%) | 360 (91%) |
| 3 | 4930 | 2020 (59%) | 1110 (79%) |
See Table 1 for abbreviations.
Two million CD8− PBMCs derived from healthy HTLV-I carriers were cultured alone, reconstituted with a half million autologous CD8+ T cells (coculture), or separated from one million of autologous CD8+ T cells by a semipermeable membrane filter (trans-well).