Table 1.

CAFC frequency in unfractionated marrow and sorted Sca-1+c-kit+Lin−stem cells from young B6 and DBA mice

CAFC d7/105 cellsCAFC d14/105cellsCAFC d21/105 cellsCAFC d28/105cellsCAFC d35/105 cells
B6 unfractionated 156 19.4 8.6 2.7 1.5 
B6 Sca-1+c-kit+Lin− nd 12 177 5 645 1 265 497 
B6 fold enrichment <1× 627× 656× 469× 331× 
DBA unfractionated 237 57 23 8.6 3.4  
DBA Sca-1+c-kit+Lin− nd 9 197 14 782 10 534 6 172 
DBA enrichment <1× 161× 642× 1 225× 1 815× 
CAFC d7/105 cellsCAFC d14/105cellsCAFC d21/105 cellsCAFC d28/105cellsCAFC d35/105 cells
B6 unfractionated 156 19.4 8.6 2.7 1.5 
B6 Sca-1+c-kit+Lin− nd 12 177 5 645 1 265 497 
B6 fold enrichment <1× 627× 656× 469× 331× 
DBA unfractionated 237 57 23 8.6 3.4  
DBA Sca-1+c-kit+Lin− nd 9 197 14 782 10 534 6 172 
DBA enrichment <1× 161× 642× 1 225× 1 815× 

nd = not detected. The frequency of Sca-1+c-kit+Lin− cells in unfractionated marrow was approximately 30 to 40 per 105 in both strains (or 0.03%-0.04% of the total population).

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