CML cell-induced morphologic apoptosis in CD56+ NK cells: protection by histamine
| Cells . | CD56+ . | CD56− . | ||
|---|---|---|---|---|
| Medium . | Histamine . | Medium . | Histamine . | |
| Lymph + CML (50)* | 64.7 | 33.3 | 7.8 | 4.3 |
| Lymph + CML (25) | 30.7 | 16.3 | 1.6 | 1.6 |
| Lymph + CML (10) | 23.2 | 6.6 | 1.3 | 0.9 |
| Cells . | CD56+ . | CD56− . | ||
|---|---|---|---|---|
| Medium . | Histamine . | Medium . | Histamine . | |
| Lymph + CML (50)* | 64.7 | 33.3 | 7.8 | 4.3 |
| Lymph + CML (25) | 30.7 | 16.3 | 1.6 | 1.6 |
| Lymph + CML (10) | 23.2 | 6.6 | 1.3 | 0.9 |
Data are expressed as the percentage of NK cells (CD56+) and T cells (CD56−) with morphologic apoptotic features, as reflected by a reduced forward and side angle scatter. The population of gated CD56− lymphocytes consisted of >80% CD3+ T cells. Final histamine concentration was 10 μmol/L. Similar results were obtained in three separate experiments.
Numbers in parentheses refer to CML cells × 103.