CML cell-induced depletion of intracellular glutathione in CD56+ NK cells: protection by histamine
| Cells . | All lymphocytes . | CD56+ . | CD56− . | |||
|---|---|---|---|---|---|---|
| Medium . | Histamine . | Medium . | Histamine . | Medium . | Histamine . | |
| Lymph + CML (50)3-150 | 35.5 | 14.9 | 71.9 | 38.4 | 14.6 | 7.1 |
| Lymph + CML (25) | 14.5 | 4.4 | 35.6 | 19.2 | 3.7 | 2.7 |
| Lymph + CML (10) | 5.4 | 3.1 | 25.3 | 9.4 | 2.6 | 1.8 |
| Cells . | All lymphocytes . | CD56+ . | CD56− . | |||
|---|---|---|---|---|---|---|
| Medium . | Histamine . | Medium . | Histamine . | Medium . | Histamine . | |
| Lymph + CML (50)3-150 | 35.5 | 14.9 | 71.9 | 38.4 | 14.6 | 7.1 |
| Lymph + CML (25) | 14.5 | 4.4 | 35.6 | 19.2 | 3.7 | 2.7 |
| Lymph + CML (10) | 5.4 | 3.1 | 25.3 | 9.4 | 2.6 | 1.8 |
Data are expressed as the percentage of NK cells (CD56+) and T cells (CD56−) with morphologic apoptotic features, as reflected by reduced intracellular content of glutathione. The population of gated CD56− lymphocytes consisted of >80% CD3+ T cells. Results obtained using lymphocytes and fMLF-activated CML cells are shown. Final histamine concentration was 10 μmol/L. Similar results were obtained in three separate experiments.
Numbers in parentheses refer to CML cells × 103.