Viability of dendritic cells after transduction with HIV-1ΔENΔV3
| DC transduction . | . | Cell labeling (%) . | |
|---|---|---|---|
| Trypan blue . | Annexin V . | ||
| Mock | 6.4 ± 5 | 16.6 ± 8 | |
| HIV-1ΔENΔV3 | MOI 5 | 6.0 ± 7 | 16.0 ± 6 |
| MOI 50 | 8.1 ± 9 | nt | |
| DC transduction . | . | Cell labeling (%) . | |
|---|---|---|---|
| Trypan blue . | Annexin V . | ||
| Mock | 6.4 ± 5 | 16.6 ± 8 | |
| HIV-1ΔENΔV3 | MOI 5 | 6.0 ± 7 | 16.0 ± 6 |
| MOI 50 | 8.1 ± 9 | nt | |
DCs were transduced as described in the legend to Figure 2. The percentages of necrotic and apoptotic cells were determined by trypan blue exclusion assay and by annexin V staining, respectively. Mean ± SD of at least 2 experiments are shown.
DCs, dendritic cells; nt, not tested.