RMA-S binding
| Name . | Sequence . | RMA-S binding . | |
|---|---|---|---|
| H-2Db (%) . | H-2Kb (%) . | ||
| WT1 peptides with motifs predictive of binding | |||
| P136-144 | SCLESQPTI | 27 | 1 |
| P221-229 | YSSDNLYQM | 1 | 1 |
| P225-233 | NLYQMTSQL | 6 | 1 |
| P228-236 | QMTSQLECM | 1 | 1 |
| P235-243 | CMTWNQMNL | 34 | 1 |
| P239-247 | NQMNLGATL | 1 | 1 |
| P329-337 | GCNKRYFKL | 1 | 1 |
| All 9mer WT1 peptides within p117-139 | |||
| P117-125 | PSQASSGQA | 2 | 7 |
| P118-126 | SQASSGQAR | 2 | 7 |
| P119-127 | QASSGQARM | 2 | 6 |
| P120-128 | ASSGQARMF | 1 | 7 |
| P121-129 | SSGQARMFP | 1 | 7 |
| P122-130 | SGQARMFPN | 1 | 7 |
| P123-131 | GQARMFPNA | 1 | 7 |
| P124-132 | QARMFPNAP | 1 | 9 |
| P125-133 | ARMFPNAPY | 1 | 8 |
| P126-134 | RMFPNAPYL | 79 | 65 |
| P127-135 | MFPNAPYLP | 2 | 8 |
| P128-136 | FPNAPYLPS | 1 | 5 |
| P129-137 | PNAPYLPSC | 1 | 4 |
| P130-138 | NAPYLPSCL | 79 | 94 |
| P131-139 | APYLPSCLE | 1 | 9 |
| Isotype control | 1 | 2 | |
| No peptide | 1 | 6 | |
| Name . | Sequence . | RMA-S binding . | |
|---|---|---|---|
| H-2Db (%) . | H-2Kb (%) . | ||
| WT1 peptides with motifs predictive of binding | |||
| P136-144 | SCLESQPTI | 27 | 1 |
| P221-229 | YSSDNLYQM | 1 | 1 |
| P225-233 | NLYQMTSQL | 6 | 1 |
| P228-236 | QMTSQLECM | 1 | 1 |
| P235-243 | CMTWNQMNL | 34 | 1 |
| P239-247 | NQMNLGATL | 1 | 1 |
| P329-337 | GCNKRYFKL | 1 | 1 |
| All 9mer WT1 peptides within p117-139 | |||
| P117-125 | PSQASSGQA | 2 | 7 |
| P118-126 | SQASSGQAR | 2 | 7 |
| P119-127 | QASSGQARM | 2 | 6 |
| P120-128 | ASSGQARMF | 1 | 7 |
| P121-129 | SSGQARMFP | 1 | 7 |
| P122-130 | SGQARMFPN | 1 | 7 |
| P123-131 | GQARMFPNA | 1 | 7 |
| P124-132 | QARMFPNAP | 1 | 9 |
| P125-133 | ARMFPNAPY | 1 | 8 |
| P126-134 | RMFPNAPYL | 79 | 65 |
| P127-135 | MFPNAPYLP | 2 | 8 |
| P128-136 | FPNAPYLPS | 1 | 5 |
| P129-137 | PNAPYLPSC | 1 | 4 |
| P130-138 | NAPYLPSCL | 79 | 94 |
| P131-139 | APYLPSCLE | 1 | 9 |
| Isotype control | 1 | 2 | |
| No peptide | 1 | 6 | |
Peptide binding to C57BL/6 murine MHC was confirmed using the RMA-S binding assay. Samples were analyzed using the Becton-Dickinson FACScalibur. The positive region was gated to contain approximately 1% of the RMA-S cells stained with isotype only. The same region was used to determine the percentage positive for class I stained samples.