Mφ cytostatic activity triggered by LPS during acute GVHD
| Treatment in vitro . | Proliferation of MDW4 targets cocultured with Mφ, % . | ||
|---|---|---|---|
| Normal . | Acute GVHD . | Syngeneic . | |
| Medium | 83.1 ± 1.3 | 82.4 ± 5.0 | 86.4 ± 1.2 |
| LPS 2.5 ng/mL | 80.9 ± 3.1 | 0.5 ± 0.1 | 82.9 ± 3.1 |
| LPS 2.5 ng/mL + IFN-γ | 1.7 ± 0.1 | 0.6 ± 0.3 | 1.3 ± 0.9 |
| LPS 2.5 ng/mL + IFN-γ + anti–IFN-γ | 83.0 ± 8.6 | 3.8 ± 0.9 | 87.1 ± 4.2 |
| LPS 2.5 ng/mL + anti–IFN-γ | 89.4 ± 4.9 | 5.6 ± 3.4 | 82.3 ± 3.7 |
| Treatment in vitro . | Proliferation of MDW4 targets cocultured with Mφ, % . | ||
|---|---|---|---|
| Normal . | Acute GVHD . | Syngeneic . | |
| Medium | 83.1 ± 1.3 | 82.4 ± 5.0 | 86.4 ± 1.2 |
| LPS 2.5 ng/mL | 80.9 ± 3.1 | 0.5 ± 0.1 | 82.9 ± 3.1 |
| LPS 2.5 ng/mL + IFN-γ | 1.7 ± 0.1 | 0.6 ± 0.3 | 1.3 ± 0.9 |
| LPS 2.5 ng/mL + IFN-γ + anti–IFN-γ | 83.0 ± 8.6 | 3.8 ± 0.9 | 87.1 ± 4.2 |
| LPS 2.5 ng/mL + anti–IFN-γ | 89.4 ± 4.9 | 5.6 ± 3.4 | 82.3 ± 3.7 |
Proliferation was calculated from 48-hour cultures of Mφ plus targets, Mφ alone, or target cells alone and expressed as the percent incorporation of [3H]TdR as follows: [(cpm Mφ + target)/[(cpm Mφ) + (cpm target)]] × 100. Acute GVHD animals were transplanted with 60 × 106 B6 cells, and syngeneic animals were transplanted with 60 × 106B6AF1 cells. Mφ were isolated on day 14 posttransplantation. Data are presented as mean ± SEM of 3 experiments. The cpm of target cells cultured in medium or medium containing 2.5 ng/mL LPS was 355 ± 19 and 359 ± 13 (×10−3), respectively.