FCS negatively affects the long-term reconstituting activity of Lin−Sca1+c−kit+ cells cultured under various conditions
| Cytokines . | Medium . | FCS . | BSA . | No.mice . | % Ly5.1+ cells in PB 4 months after transplantation . |
|---|---|---|---|---|---|
| Fresh cells | NA | NA | NA | 4 | 36.75 ± 18.37 |
| KFM3 | X-Vivo 15 | — | 1% | 5 | 64.02 ± 13.76 |
| KFM3 | IMDM | 20% | — | 3 | 0.74 ± 0.65 |
| KFM3 | X-Vivo 15 | 20% | 1% | 4 | 0.08 ± 0.01 |
| KFM3 | IMDM | 20% | 1% | 5 | 0.06 ± 0.03 |
| K6,11E34-150 | X-Vivo 15 | — | 1% | 5 | 60.2 ± 14.74 |
| K6,11E3 | IMDM | 20% | — | 3 | 0.57 ± 0.30 |
| Cytokines . | Medium . | FCS . | BSA . | No.mice . | % Ly5.1+ cells in PB 4 months after transplantation . |
|---|---|---|---|---|---|
| Fresh cells | NA | NA | NA | 4 | 36.75 ± 18.37 |
| KFM3 | X-Vivo 15 | — | 1% | 5 | 64.02 ± 13.76 |
| KFM3 | IMDM | 20% | — | 3 | 0.74 ± 0.65 |
| KFM3 | X-Vivo 15 | 20% | 1% | 4 | 0.08 ± 0.01 |
| KFM3 | IMDM | 20% | 1% | 5 | 0.06 ± 0.03 |
| K6,11E34-150 | X-Vivo 15 | — | 1% | 5 | 60.2 ± 14.74 |
| K6,11E3 | IMDM | 20% | — | 3 | 0.57 ± 0.30 |
LSK Ly5.1+ cells were cultured for 10 days in various media and cytokines. Five hundred uncultured LSK cells (control), or the expansion equivalent of 500 LSK cells, were injected into lethally irradiated C57Bl/6 recipients (Ly5.2+) together with 200 000 fresh Ly5.2+ BM cells. PB from transplanted mice was analyzed 4 months after transplantation for donor-derived hematopoiesis. Results are shown are means ± SEM. NA, not applicable.
K6,11E3 = mKL + hIL-6 + hIL-11 + hEPO + mIL-3.