Table 1. MetHb formation in erythrocytes... | American Society of Hematology

Table 1.

MetHb formation in erythrocytes exposed to a constant flux of H₂O₂

Experiment	Rate	% metHb (wild-type cells)			% metHb (GSHPx-1-deficient cells)
Experiment	Rate	0 h	5 h	Increase	0 h	5 h	Increase
1	0.12	0.67	4.20	3.53	1.0	4.1	3.10
2	0.12	1.3	1.6	0.30	1.8	1.8	0.00
3	0.12	1.4	1.7	0.30	1.8	1.8	0.00
4	0.62	1.6	1.7	0.10	1.6	1.7	0.10
5	0.62	1.6	1.7	0.10	1.6	1.9	0.30
6	1.23	1.7	1.8	0.10	1.4	3.9	2.50
7	1.23	1.2	1.4	0.20	1.6	1.6	0.00
8	6.15	1.3	1.9	0.60	1.5	2.5	1.00

Experiment	Rate	% metHb (wild-type cells)			% metHb (GSHPx-1-deficient cells)
Experiment	Rate	0 h	5 h	Increase	0 h	5 h	Increase
1	0.12	0.67	4.20	3.53	1.0	4.1	3.10
2	0.12	1.3	1.6	0.30	1.8	1.8	0.00
3	0.12	1.4	1.7	0.30	1.8	1.8	0.00
4	0.62	1.6	1.7	0.10	1.6	1.7	0.10
5	0.62	1.6	1.7	0.10	1.6	1.9	0.30
6	1.23	1.7	1.8	0.10	1.4	3.9	2.50
7	1.23	1.2	1.4	0.20	1.6	1.6	0.00
8	6.15	1.3	1.9	0.60	1.5	2.5	1.00

Red cells were exposed to a continuous flux of H₂O₂ generated by a GO/glucose system in the presence of gentamicin. The metHb values at the beginning of the exposure period (0 hours) and after 5 hours are shown.

Rate, μmol/L H₂O₂ produced per minute.

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