Percentage of colony types from CFU assays and cell types from differential blood counts of founder lines #7 and #8*
| . | Founder line #7 . | Founder line #8 . | Wild type . |
|---|---|---|---|
| Colonies from CFU assay (%) | |||
| GM | 65.5 ± 8.1 | 56.2 ± 7.1 | 57.8 ± 7.4 |
| Macrophages | 21.1 ± 9.6 | 27.8 ± 9.0 | 26.0 ± 9.0 |
| Mix | 9.4 ± 2.8 | 8.0 ± 0.8 | 10.9 ± 3.2 |
| Erythrocytes | 4.0 ± 2.1 | 7.2 ± 1.7 | 5.6 ± 2.4 |
| Differential blood counts (%) | |||
| Neutrophils | 20.7 ± 7.2 | 16.4 ± 8.8 | 18.8 ± 3.9 |
| Lymphocytes | 77.6 ± 6.8 | 81.8 ± 9.1 | 80.2 ± 3.9 |
| Monocytes | 1.7 ± 0.8 | 1.8 ± 1.1 | 1.0 ± 0.0 |
| . | Founder line #7 . | Founder line #8 . | Wild type . |
|---|---|---|---|
| Colonies from CFU assay (%) | |||
| GM | 65.5 ± 8.1 | 56.2 ± 7.1 | 57.8 ± 7.4 |
| Macrophages | 21.1 ± 9.6 | 27.8 ± 9.0 | 26.0 ± 9.0 |
| Mix | 9.4 ± 2.8 | 8.0 ± 0.8 | 10.9 ± 3.2 |
| Erythrocytes | 4.0 ± 2.1 | 7.2 ± 1.7 | 5.6 ± 2.4 |
| Differential blood counts (%) | |||
| Neutrophils | 20.7 ± 7.2 | 16.4 ± 8.8 | 18.8 ± 3.9 |
| Lymphocytes | 77.6 ± 6.8 | 81.8 ± 9.1 | 80.2 ± 3.9 |
| Monocytes | 1.7 ± 0.8 | 1.8 ± 1.1 | 1.0 ± 0.0 |
Mice positive for both the murine mammary tumor virus-tet-controlled transcriptional activator (MMTV-tTA) and pUHD-AML1/ETO constructs as well as wild-type mice were killed and the bone marrow was harvested. Colony forming unit (CFU) assays were performed and the colonies were counted. The percentage of each colony type is shown. In addition, peripheral blood was harvested from the tail and blood smears were made. The smears were stained with Wright-Giemsa and 100 to 200 cells of each slide were counted. The percentage of each cell type is shown.