Thrombopoietin-induced megakaryocyte colony formation is inhibited by IFN-α
| . | MK colony no. . | . |
|---|---|---|
| 10 ng/mL TPO | 102 ± 11 | |
| 10 ng/mL TPO + 500 U/mL IFN-α | 65 ± 4 | P < .03 |
| 3 ng/mL TPO | 74 ± 7 | |
| 3 ng/mL TPO + 500 U/mL IFN-α | 45 ± 7 | P < .02 |
| . | MK colony no. . | . |
|---|---|---|
| 10 ng/mL TPO | 102 ± 11 | |
| 10 ng/mL TPO + 500 U/mL IFN-α | 65 ± 4 | P < .03 |
| 3 ng/mL TPO | 74 ± 7 | |
| 3 ng/mL TPO + 500 U/mL IFN-α | 45 ± 7 | P < .02 |
Murine marrow cells were cultured in agar in the presence of 3 to 10 ng/mL TPO with or without 500 U/mL IFN-α. Culture plates were stained for AChE after 5 days of culture, and MK colonies were enumerated by inverted microscopy. Results represent the mean (±SEM) number of MK colonies per 2 × 105 marrow cells plated in three separate experiments.