Cell and progenitor counts after mobilization in L-selectin-deficient mice
| . | Blood CFU-Cs (per mL) . | BM CFU-Cs (×103/femur) . | Spleen CFU-Cs (×103/spleen) . | WBC (per μL) . | BMNC (×106/femur) . | Platelets (×103/μL) . |
|---|---|---|---|---|---|---|
| WT PBS | 19 ± 6 | 72.5 ± 3.8 | 9.8 ± 0.8 | 2 700 ± 441 | 17.4 ± 0.9 | 853 ± 49 |
| WT fucoidin | 271 ± 61* | 65.4 ± 2.5 | 10.7 ± 1.8 | 22 510 ± 3 141* | 14.8 ± 0.7* | 435 ± 45* |
| L−/− PBS | 19 ± 12 | 62.1 ± 5.1 | 8.4 ± 0.9 | 3 460 ± 533 | 16.2 ± 0.6 | 837 ± 44 |
| L−/− fucoidin | 269 ± 63* | 70.8 ± 4.9 | 11.4 ± 1.3 | 19 033 ± 1 777* | 13.7 ± 0.5* | 448 ± 46* |
| . | Blood CFU-Cs (per mL) . | BM CFU-Cs (×103/femur) . | Spleen CFU-Cs (×103/spleen) . | WBC (per μL) . | BMNC (×106/femur) . | Platelets (×103/μL) . |
|---|---|---|---|---|---|---|
| WT PBS | 19 ± 6 | 72.5 ± 3.8 | 9.8 ± 0.8 | 2 700 ± 441 | 17.4 ± 0.9 | 853 ± 49 |
| WT fucoidin | 271 ± 61* | 65.4 ± 2.5 | 10.7 ± 1.8 | 22 510 ± 3 141* | 14.8 ± 0.7* | 435 ± 45* |
| L−/− PBS | 19 ± 12 | 62.1 ± 5.1 | 8.4 ± 0.9 | 3 460 ± 533 | 16.2 ± 0.6 | 837 ± 44 |
| L−/− fucoidin | 269 ± 63* | 70.8 ± 4.9 | 11.4 ± 1.3 | 19 033 ± 1 777* | 13.7 ± 0.5* | 448 ± 46* |
Wild-type (WT) and L-selectin-deficient (L−/−) mice were treated with 2 sequential doses of fucoidan (25 mg/kg). Six hours after the initial dose, blood, bone marrow (BM), and spleen were harvested. Blood nucleated cell (white blood cell [WBC]), platelets, and bone marrow nucleated cell (BMNC) counts were determined using an automated cell counter. An aliquot of cells was plated in methylcellulose media and read 8 days after cultivation to assess progenitor counts.
P < .05; n = 9-10 mice, except spleen where n = 7.
CFU-Cs, colony-forming units in culture.