Comparison of the engraftment level and the efficiency of gene transfer to adult human BM cells capable of repopulating NOD/SCID-β2M−/− mice using 2 slightly different 6-day transduction protocols
| No. of cells per mouse* . | Protocol A . | Protocol B . | ||
|---|---|---|---|---|
| % human CD45/71+ cells . | % GFP+ human CD45/71+ cells . | % human CD45/71+ cells . | % GFP+ human CD45/71+ cells . | |
| 53 000 | 1.0 | 3 | 2.1 | 2 |
| 53 000 | 1.2 | 19 | 0.3 | 9 |
| 53 000 | 0.3 | 2 | 1.1 | 13 |
| 53 000 | 1.0 | 4 | ||
| 80 000 | 1.9 | 23 | 2.9 | 13 |
| 80 000 | 11.0 | 16 | 4.3 | 20 |
| 80 000 | 1.0 | 37 | 2.5 | 21 |
| 80 000 | 1.5 | 33 | 2.1 | 12 |
| Mean ± SEM | 2.4 ± 1.2 | 17 ± 5 | 2.2 ± 0.5 | 13 ± 2 |
| No. of cells per mouse* . | Protocol A . | Protocol B . | ||
|---|---|---|---|---|
| % human CD45/71+ cells . | % GFP+ human CD45/71+ cells . | % human CD45/71+ cells . | % GFP+ human CD45/71+ cells . | |
| 53 000 | 1.0 | 3 | 2.1 | 2 |
| 53 000 | 1.2 | 19 | 0.3 | 9 |
| 53 000 | 0.3 | 2 | 1.1 | 13 |
| 53 000 | 1.0 | 4 | ||
| 80 000 | 1.9 | 23 | 2.9 | 13 |
| 80 000 | 11.0 | 16 | 4.3 | 20 |
| 80 000 | 1.0 | 37 | 2.5 | 21 |
| 80 000 | 1.5 | 33 | 2.1 | 12 |
| Mean ± SEM | 2.4 ± 1.2 | 17 ± 5 | 2.2 ± 0.5 | 13 ± 2 |
CD34+CD38− were stimulated for 3 days (protocol A) or 4 days (protocol B) with FL + SF + IL-3 + HIL-6 and then exposed to VCM for 72 hours (3×) or 48 hours (1×), respectively. At the end of this period, the cells were harvested and injected into sublethally irradiated NOD/SCID-β2M−/− mice. The mice were then analyzed 6 to 8 weeks later as described in the footnote to Table 1.
This refers to the number of CD34+CD38−cells at the start of the transduction protocol, the progeny of which were injected into each NOD/SCID-β2M−/−mouse.