Effects of anti-PSGL-1 antibodies, P-selectin-IgG chimera, and anti-L-selectin antibody on lymphocyte adherence to KG1a cells
| Blocking treatments . | Percentage control binding* (SEM) . |
|---|---|
| Anti–PSGL-1 monoclonal antibodies (20 μg/mL of each in a cocktail) | 103.0 (7.0) |
| P-selectin–IgG chimera, 50 μg/mL | 97.6 (7.4) |
| Anti–L-selectin antibody | 1.9 (0.6)† |
| Blocking treatments . | Percentage control binding* (SEM) . |
|---|---|
| Anti–PSGL-1 monoclonal antibodies (20 μg/mL of each in a cocktail) | 103.0 (7.0) |
| P-selectin–IgG chimera, 50 μg/mL | 97.6 (7.4) |
| Anti–L-selectin antibody | 1.9 (0.6)† |
Number of lymphocytes adherent to confluent area of KG1a were counted by light microscopy using an ocular grid under 100× magnification (quantified a minimum of 3 fields per slide, 3 slides per experiment, 3 separate experiments). Results are presented as percentage binding compared with corresponding isotype MoAb-treated control KG1a cytospin preparations.
Similar abrogation of binding was observed in assays performed in the presence of EDTA and utilizing PMA-treated lymphocytes (see text for details).