L-selectin ligand activity of KG1a cells after protease or phospholipase C treatment
| Enzymatic treatments . | Percentage control binding* (SEM) . |
|---|---|
| O-sialoglycoprotease | 98.4 (2.3) |
| Mocarhagin | 98.9 (6.3) |
| Bromelain | 3.8 (0.4)† |
| Chymotrypsin | 6.7 (0.7)† |
| Pronase | 9.4 (1.3)† |
| Porcine pancreas elastase | 47.3 (17.6)† |
| Human neutrophil elastase | 25.4 (13.5)† |
| PI-PLC | 98.1 (2.0) |
| Enzymatic treatments . | Percentage control binding* (SEM) . |
|---|---|
| O-sialoglycoprotease | 98.4 (2.3) |
| Mocarhagin | 98.9 (6.3) |
| Bromelain | 3.8 (0.4)† |
| Chymotrypsin | 6.7 (0.7)† |
| Pronase | 9.4 (1.3)† |
| Porcine pancreas elastase | 47.3 (17.6)† |
| Human neutrophil elastase | 25.4 (13.5)† |
| PI-PLC | 98.1 (2.0) |
Number of lymphocytes adherent to confluent area of KG1a were counted by light microscopy using an ocular grid under 100× magnification (quantified a minimum of 3 fields per slide, 3 slides per experiment, 3 separate experiments). Results are presented as percentage binding compared with corresponding buffer-treated control KG1a cytospin preparations.
Statistically significant (paired t test;P < .01) difference compared with enzyme buffer alone group.