L-selectin ligand activity of KG1a cells treated with inhibitors of N-linked glycosylation
| KG1a treatment4-150 . | Percentage control adhesion (SEM)4-151 . |
|---|---|
| Neuraminidase (1 h) | 1.0 (0.7)‡ |
| Neuraminidase (1-h then 24-h recovery) | 99.3 (1.3) |
| Tunicamycin alone | 96.5 (7.4) |
| Deoxymannojirimycin alone | 107.5 (6.7) |
| Swainsonine alone | 95.5 (10.3) |
| Neuraminidase + tunicamycin | 1.0 (0.7)‡ |
| Neuraminidase + deoxymannojirimycin | 22.5 (0.4)‡ |
| Neuraminidase + swainsonine | 26.6 (2.5)‡ |
| KG1a treatment4-150 . | Percentage control adhesion (SEM)4-151 . |
|---|---|
| Neuraminidase (1 h) | 1.0 (0.7)‡ |
| Neuraminidase (1-h then 24-h recovery) | 99.3 (1.3) |
| Tunicamycin alone | 96.5 (7.4) |
| Deoxymannojirimycin alone | 107.5 (6.7) |
| Swainsonine alone | 95.5 (10.3) |
| Neuraminidase + tunicamycin | 1.0 (0.7)‡ |
| Neuraminidase + deoxymannojirimycin | 22.5 (0.4)‡ |
| Neuraminidase + swainsonine | 26.6 (2.5)‡ |
KG1a cells were cultured in the presence of tunicamycin (15 μg/mL), deoxymannojirimycin (0.4 mg/mL), or swainsonine (40 μg/mL) for 24 hours after a 1-hour (preculture) treatment with Vibrio cholera neuraminidase (0.1 U/mL). Cytospin preparations of these cells were then subjected to Stamper-Woodruff assay as detailed in the text. L-selectin–specific lymphocyte binding to cytospins was quantified by light microscopy.
Values represent mean percentage lymphocyte adherence (SE of the mean) compared with untreated control group from 3 independent experiments. Adherent lymphocytes were counted from 4 fields on each cytospin in triplicate slides using an optical grid under 100× magnification.
Statistical significance (paired t test;P < .01) compared with neuraminidase (1-hour then 24-hour recovery) group.