L-selectin ligand activity of KG1a cells treated with N-glycosidases
| N-glycosidase treatment of KG1a membrane preparation5-150 . | Percentage control adhesion (SEM)5-151 . |
|---|---|
| Endoglycosidase H | 105.5 (2.5) |
| Endoglycosidase F2 | 66.9 (0.1)5-152 |
| N-glycosidase F | 16.0 (0.7)5-153 |
| N-glycosidase treatment of KG1a membrane preparation5-150 . | Percentage control adhesion (SEM)5-151 . |
|---|---|
| Endoglycosidase H | 105.5 (2.5) |
| Endoglycosidase F2 | 66.9 (0.1)5-152 |
| N-glycosidase F | 16.0 (0.7)5-153 |
KG1a membrane preparations (0.2 mg/mL) were incubated with either Endoglycosidase H (50 mU/mL), Endoglycosidase F2 (4 mU/mL), N-glycosidase F (8 U/mL), or respective buffers (controls) for 24 hours at 37°C, then spotted onto glass slides (1 μg per spot) and subjected to Stamper-Woodruff assays as detailed in the text. L-selectin-specific lymphocyte binding to spotted samples was quantified by light microscopy.
Values represent mean percentage adherence (SE of the mean) compared with buffer control groups from 3 independent experiments. Adherent lymphocytes were counted from 4 fields on each spot in triplicate slides using an optical grid under 100× magnification.
Statistical significance (Paired t test;P = .03) compared with buffer control group.
Statistical significance (Paired t test; P< .01) compared with buffer control group.