DNA oligomers used to create the β-globin gene variants by overlap-extension PCR
| Oligomer* . | Orientation† . | Sequence (5′ → 3′)‡ . | Position1-153 . |
|---|---|---|---|
| β5(ATC)b | − | TCTCCTCCTAAGTCAGGTG | |
| β5(TAG)c | + | CACCTGACTTAGGAGGAGA | 56 |
| β15(ATC)b | − | CCTTGCCCTACAGGGCAG | |
| β15(TAG)c | + | CTGCCCTGTAGGGCAAGG | 86 |
| β15(ATT)b | − | CCTTGCCTTACAGGGCAG | |
| β15(TAA)c | + | CTGCCCTGTAAGGCAAGG | 86 |
| β17(ATC)b | − | CGTTCACCTAGCCCCAC | |
| β17(TAG)c | + | GTGGGGCTAGGTGAACG | 94 |
| β26(ATC)b | − | CCAGGGCCTAACCACCAA | |
| β26(TAG)c | + | TTGGTGGTTAGGCCCTGG | 120 |
| Oligomer* . | Orientation† . | Sequence (5′ → 3′)‡ . | Position1-153 . |
|---|---|---|---|
| β5(ATC)b | − | TCTCCTCCTAAGTCAGGTG | |
| β5(TAG)c | + | CACCTGACTTAGGAGGAGA | 56 |
| β15(ATC)b | − | CCTTGCCCTACAGGGCAG | |
| β15(TAG)c | + | CTGCCCTGTAGGGCAAGG | 86 |
| β15(ATT)b | − | CCTTGCCTTACAGGGCAG | |
| β15(TAA)c | + | CTGCCCTGTAAGGCAAGG | 86 |
| β17(ATC)b | − | CGTTCACCTAGCCCCAC | |
| β17(TAG)c | + | GTGGGGCTAGGTGAACG | 94 |
| β26(ATC)b | − | CCAGGGCCTAACCACCAA | |
| β26(TAG)c | + | TTGGTGGTTAGGCCCTGG | 120 |
The number in the oligomer designations indicates the codon in which the nonsense mutation was introduced.
Minus signs indicate antisense; plus signs indicate sense.
Underlined sequences indicate the nonsense codons created by overlap-extension PCR.
Distance (in nucleotides) between the oligomer sequence and the transcriptional initiation site of the human β-globin gene.