Quantitation of eGFP+ donor CD4+ T cells in bm12 recipient mice
| . | Day 6 Liver* . | Day 6 GI Tract . | Day 6 Lung2-160 . | Day 12 Liver . | Day 12 GI Tract . | Day 12 Lung . |
|---|---|---|---|---|---|---|
| −/− | 75 ± 6 | 30 ± 2 | 65 ± 6 | NA | NA | NA |
| +/+ | 25 ± 4 | 25 ± 6 | 28 ± 4 | 300 ± 60 | 100 ± 30 | 350 ± 40 |
| . | Day 6 Liver* . | Day 6 GI Tract . | Day 6 Lung2-160 . | Day 12 Liver . | Day 12 GI Tract . | Day 12 Lung . |
|---|---|---|---|---|---|---|
| −/− | 75 ± 6 | 30 ± 2 | 65 ± 6 | NA | NA | NA |
| +/+ | 25 ± 4 | 25 ± 6 | 28 ± 4 | 300 ± 60 | 100 ± 30 | 350 ± 40 |
The SD is given as × ± 103 cells. Day 12 samples were not available (NA) from bm12 recipients after the transfer of MIP-1α−/− splenocytes, as all mice died by day 10 due to severe GVHD. The number of donor cells (eGFP+) CD4+ and CD8+ T cells was derived by multiplying the percent of those cells by flow cytometry by the total number of cells present in a comparable section of tissue. The total number of cells was counted manually using a hemocytometer. The data were pooled from 3 separate experiments using 3 bm1 and bm12 mice in each group. The data were expressed as the mean of donor CD4+ or CD8+ T cells, with SD for each organ. SD was analyzed using the Student t test.
P = .01.
P = .03.